Evaluation of three diagnostic methods, including real-time PCR, for detection of Dientamoeba fragilis in stool specimens

Stark, D; Beebe, N; Marriott, D; Ellis, J; Harkness, J

Evaluation of three diagnostic methods, including real-time PCR, for detection of Dientamoeba fragilis in stool specimens

Stark, D Beebe, N

Marriott, D Ellis, J

Harkness, J

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Publication Type:: Journal Article
Citation:: Journal of Clinical Microbiology, 2006, 44 (1), pp. 232 - 235
Issue Date:: 2006-01-01

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Field	Value	Language
dc.contributor.author	Stark, D	en_US
dc.contributor.author	Beebe, N https://orcid.org/0000-0003-2877-0691	en_US
dc.contributor.author	Marriott, D	en_US
dc.contributor.author	Ellis, J https://orcid.org/0000-0001-7328-4831	en_US
dc.contributor.author	Harkness, J	en_US
dc.date.issued	2006-01-01	en_US
dc.identifier.citation	Journal of Clinical Microbiology, 2006, 44 (1), pp. 232 - 235	en_US
dc.identifier.issn	0095-1137	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3787
dc.description.abstract	Dientamoeba fragilis is a protozoan parasite of humans that infects the mucosa of the large intestine and is associated with gastrointestinal disease. We developed a 5′ nuclease (TaqMan)-based real-time PCR assay, targeting the small subunit rRNA gene, for the detection of D. fragilis in human stool specimens and compared its sensitivity and specificity to conventional PCR and microscopic examination by a traditional modified iron-hematoxylin staining procedure. Real-time PCR exhibited 100% sensitivity and specificity. Copyright © 2006, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation.ispartof	Journal of Clinical Microbiology	en_US
dc.relation.isbasedon	10.1128/JCM.44.1.232-235.2006	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Feces	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Dientamoeba	en_US
dc.subject.mesh	Dientamoebiasis	en_US
dc.subject.mesh	Diarrhea	en_US
dc.subject.mesh	DNA, Protozoan	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Genes, rRNA	en_US
dc.title	Evaluation of three diagnostic methods, including real-time PCR, for detection of Dientamoeba fragilis in stool specimens	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	44	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	44	en_US

Abstract:

Dientamoeba fragilis is a protozoan parasite of humans that infects the mucosa of the large intestine and is associated with gastrointestinal disease. We developed a 5′ nuclease (TaqMan)-based real-time PCR assay, targeting the small subunit rRNA gene, for the detection of D. fragilis in human stool specimens and compared its sensitivity and specificity to conventional PCR and microscopic examination by a traditional modified iron-hematoxylin staining procedure. Real-time PCR exhibited 100% sensitivity and specificity. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/3787