Ribosomal DNA spacer genotypes of the anopheles bancroftii group (Diptera: Culicidae) from Australia and Papua New Guinea

Beebe, NW; Maung, J; Van Den Hurk, AF; Ellis, JT; Cooper, RD

Ribosomal DNA spacer genotypes of the anopheles bancroftii group (Diptera: Culicidae) from Australia and Papua New Guinea

Beebe, NW

Maung, J Van Den Hurk, AF Ellis, JT

Cooper, RD

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Publication Type:: Journal Article
Citation:: Insect Molecular Biology, 2001, 10 (5), pp. 407 - 413
Issue Date:: 2001-11-07

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Field	Value	Language
dc.contributor.author	Beebe, NW https://orcid.org/0000-0003-2877-0691	en_US
dc.contributor.author	Maung, J	en_US
dc.contributor.author	Van Den Hurk, AF	en_US
dc.contributor.author	Ellis, JT https://orcid.org/0000-0001-7328-4831	en_US
dc.contributor.author	Cooper, RD	en_US
dc.date.issued	2001-11-07	en_US
dc.identifier.citation	Insect Molecular Biology, 2001, 10 (5), pp. 407 - 413	en_US
dc.identifier.issn	0962-1075	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3791
dc.description.abstract	Mosquitoes of the Anopheles bancroftii group collected from Northern Australia and Papua New Guinea (PNG) were investigated for sequence variation within the ribosomal DNA ITS2. Wing fringe morphology originally used to identify members of this group was compared to genotypes identified by restriction fragment length polymorphism analysis (RFLP) and heteroduplex analysis (HDA) of the rDNA ITS2. Members of this group separated into four RFLP genotypes (A, B, C and D) with some genotypes displaying wing fringe polymorphisms. Heteroduplex analysis of the ITS2 within and between populations identified genotype A as containing two geographically separate ITS2 sequences: A1 from the Northern Territory of Australia and A2 from Queensland and the Western Province of PNG. Genotypes B and C and genotypes C and D were found sympatric and appeared to be evolving independently suggesting the possibility of cryptic species. Genotype C contained two ITS2 sequence types within the genome.	en_US
dc.relation.ispartof	Insect Molecular Biology	en_US
dc.relation.isbasedon	10.1046/j.0962-1075.2001.00278.x	en_US
dc.subject.classification	Entomology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Anopheles	en_US
dc.subject.mesh	Australia	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	DNA, Ribosomal	en_US
dc.subject.mesh	DNA, Ribosomal Spacer	en_US
dc.subject.mesh	Genetic Variation	en_US
dc.subject.mesh	Genotype	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Nucleic Acid Heteroduplexes	en_US
dc.subject.mesh	Papua New Guinea	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Polymorphism, Genetic	en_US
dc.subject.mesh	Polymorphism, Restriction Fragment Length	en_US
dc.subject.mesh	Sequence Alignment	en_US
dc.subject.mesh	Sequence Homology, Nucleic Acid	en_US
dc.subject.mesh	Wings, Animal	en_US
dc.title	Ribosomal DNA spacer genotypes of the anopheles bancroftii group (Diptera: Culicidae) from Australia and Papua New Guinea	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	10	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	10	en_US

Abstract:

Mosquitoes of the Anopheles bancroftii group collected from Northern Australia and Papua New Guinea (PNG) were investigated for sequence variation within the ribosomal DNA ITS2. Wing fringe morphology originally used to identify members of this group was compared to genotypes identified by restriction fragment length polymorphism analysis (RFLP) and heteroduplex analysis (HDA) of the rDNA ITS2. Members of this group separated into four RFLP genotypes (A, B, C and D) with some genotypes displaying wing fringe polymorphisms. Heteroduplex analysis of the ITS2 within and between populations identified genotype A as containing two geographically separate ITS2 sequences: A1 from the Northern Territory of Australia and A2 from Queensland and the Western Province of PNG. Genotypes B and C and genotypes C and D were found sympatric and appeared to be evolving independently suggesting the possibility of cryptic species. Genotype C contained two ITS2 sequence types within the genome.

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http://hdl.handle.net/10453/3791