Bacillus subtilis YabA is involved in determining the timing and synchrony of replication initiation

Hayashi, M; Ogura, Y; Harry, EJ; Ogasawara, N; Moriya, S

Bacillus subtilis YabA is involved in determining the timing and synchrony of replication initiation

Hayashi, M Ogura, Y Harry, EJ

Ogasawara, N Moriya, S

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Publication Type:: Journal Article
Citation:: FEMS Microbiology Letters, 2005, 247 (1), pp. 73 - 79
Issue Date:: 2005-06-01

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Field	Value	Language
dc.contributor.author	Hayashi, M	en_US
dc.contributor.author	Ogura, Y	en_US
dc.contributor.author	Harry, EJ https://orcid.org/0000-0003-0858-9473	en_US
dc.contributor.author	Ogasawara, N	en_US
dc.contributor.author	Moriya, S	en_US
dc.date.available	2005-04-19	en_US
dc.date.issued	2005-06-01	en_US
dc.identifier.citation	FEMS Microbiology Letters, 2005, 247 (1), pp. 73 - 79	en_US
dc.identifier.issn	0378-1097	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3831
dc.description.abstract	It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (β subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (τ subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	FEMS Microbiology Letters	en_US
dc.relation.isbasedon	10.1016/j.femsle.2005.04.028	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Bacillus subtilis	en_US
dc.subject.mesh	DNA Helicases	en_US
dc.subject.mesh	DNA-Directed DNA Polymerase	en_US
dc.subject.mesh	DNA Polymerase III	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	DNA-Binding Proteins	en_US
dc.subject.mesh	Trans-Activators	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	Flow Cytometry	en_US
dc.subject.mesh	DNA Replication	en_US
dc.title	Bacillus subtilis YabA is involved in determining the timing and synchrony of replication initiation	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	247	en_US
utslib.for	060503 Microbial Genetics	en_US
utslib.for	060501 Bacteriology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	Auckland
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	247	en_US

Abstract:

It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (β subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (τ subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/3831