Reduction in transplacental transmission of Neospora caninum in outbred mice by vaccination

Miller, C; Quinn, H; Ryce, C; Reichel, MP; Ellis, JT

Reduction in transplacental transmission of Neospora caninum in outbred mice by vaccination

Miller, C

Quinn, H Ryce, C Reichel, MP

Ellis, JT

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Publication Type:: Journal Article
Citation:: International Journal for Parasitology, 2005, 35 (7), pp. 821 - 828
Issue Date:: 2005-06-01

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Field	Value	Language
dc.contributor.author	Miller, C https://orcid.org/0000-0001-6071-5102	en_US
dc.contributor.author	Quinn, H	en_US
dc.contributor.author	Ryce, C	en_US
dc.contributor.author	Reichel, MP https://orcid.org/0000-0002-9792-8336	en_US
dc.contributor.author	Ellis, JT https://orcid.org/0000-0001-7328-4831	en_US
dc.date.available	2005-03-07	en_US
dc.date.issued	2005-06-01	en_US
dc.identifier.citation	International Journal for Parasitology, 2005, 35 (7), pp. 821 - 828	en_US
dc.identifier.issn	0020-7519	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3900
dc.description.abstract	Infection with the protozoan parasite Neospora caninum is an important cause of abortion in cattle. A major source of infection is transplacental transfer of the parasite from mother to offspring during pregnancy. This study describes investigations on the immunisation of outbred Qs mice before pregnancy with live or a crude lysate of N. caninum (NC-Nowra isolate) to prevent transplacental transfer of a challenge infection administered during pregnancy. Parasites present in the brains of pups from mice challenged with N. caninum (NC-Liverpool) were detected by PCR. Injection of live NC-Nowra tachyzoites before pregnancy dramatically reduced transplacental transfer from 75 to 0.8% in one experiment and from 76 to 8% in a second experiment. Injection of a crude lysate of NC-Nowra tachyzoites reduced transplacental transfer from 67 to 53% in one experiment and from 76 to 63% in a second experiment. Analysis of N. caninum-specific IgG1 and IgG2a antibody levels prior to pregnancy and challenge showed that NC-Nowra lysate induced a response skewed towards IgG1 whereas live parasites induced both IgG1 and IgG2a antibodies. After pregnancy and a challenge infection, a similar IgG1/IgG2a response was seen in all challenged groups. These results provide further positive support for the hypothesis that transplacental transmission of this parasite is preventable by vaccination. © 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.	en_US
dc.relation.ispartof	International Journal for Parasitology	en_US
dc.relation.isbasedon	10.1016/j.ijpara.2005.03.006	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Placenta	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Neospora	en_US
dc.subject.mesh	Pregnancy Complications, Parasitic	en_US
dc.subject.mesh	Coccidiosis	en_US
dc.subject.mesh	Disease Models, Animal	en_US
dc.subject.mesh	Immunoglobulin G	en_US
dc.subject.mesh	Protozoan Vaccines	en_US
dc.subject.mesh	Antibodies, Protozoan	en_US
dc.subject.mesh	Postpartum Period	en_US
dc.subject.mesh	Pregnancy	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Infectious Disease Transmission, Vertical	en_US
dc.title	Reduction in transplacental transmission of Neospora caninum in outbred mice by vaccination	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	35	en_US
utslib.for	070708 Veterinary Parasitology	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0608 Zoology	en_US
utslib.for	0707 Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	35	en_US

Abstract:

Infection with the protozoan parasite Neospora caninum is an important cause of abortion in cattle. A major source of infection is transplacental transfer of the parasite from mother to offspring during pregnancy. This study describes investigations on the immunisation of outbred Qs mice before pregnancy with live or a crude lysate of N. caninum (NC-Nowra isolate) to prevent transplacental transfer of a challenge infection administered during pregnancy. Parasites present in the brains of pups from mice challenged with N. caninum (NC-Liverpool) were detected by PCR. Injection of live NC-Nowra tachyzoites before pregnancy dramatically reduced transplacental transfer from 75 to 0.8% in one experiment and from 76 to 8% in a second experiment. Injection of a crude lysate of NC-Nowra tachyzoites reduced transplacental transfer from 67 to 53% in one experiment and from 76 to 63% in a second experiment. Analysis of N. caninum-specific IgG1 and IgG2a antibody levels prior to pregnancy and challenge showed that NC-Nowra lysate induced a response skewed towards IgG1 whereas live parasites induced both IgG1 and IgG2a antibodies. After pregnancy and a challenge infection, a similar IgG1/IgG2a response was seen in all challenged groups. These results provide further positive support for the hypothesis that transplacental transmission of this parasite is preventable by vaccination. © 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/3900