Prevalence of Neospora caninum infection in Australian (NSW) dairy cattle estimated by a newly validated ELISA for milk

Hall, CA; Reichel, MP; Ellis, JT

Prevalence of Neospora caninum infection in Australian (NSW) dairy cattle estimated by a newly validated ELISA for milk

Hall, CA Reichel, MP

Ellis, JT

Permalink

Publication Type:: Journal Article
Citation:: Veterinary Parasitology, 2006, 142 (1-2), pp. 173 - 178
Issue Date:: 2006-11-30

Closed Access

	Filename	Description	Size
	2006004324.pdf		923.86 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Hall, CA	en_US
dc.contributor.author	Reichel, MP https://orcid.org/0000-0002-9792-8336	en_US
dc.contributor.author	Ellis, JT https://orcid.org/0000-0001-7328-4831	en_US
dc.date.available	2006-06-16	en_US
dc.date.issued	2006-11-30	en_US
dc.identifier.citation	Veterinary Parasitology, 2006, 142 (1-2), pp. 173 - 178	en_US
dc.identifier.issn	0304-4017	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3943
dc.description.abstract	Aim: To determine the performance characteristics of an Institut Pourquier (IP) enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against Neospora caninum in bovine milk and subsequent determination of the prevalence of N. caninum infection in New South Wales (NSW) dairy cattle. Methods: Matching serum and milk samples from 93 cattle were assayed in two commercially available ELISAs for the detection of anti-N. caninum antibodies. Serum test results of one ELISA (IDEXX) were used to determine the N. caninum infection status of the cattle. Optimised cut-off values for the IP ELISA using milk samples were determined by two-graph receiver operating characteristic (TG-ROC) analysis and then applied to a representative sample of 398 milk samples from dairy herds around NSW. Results: When this ELISA was applied to a representative collection of 398 milk samples from dairy cattle across NSW it demonstrated a 21.1% prevalence of N. caninum infection in those cattle. From the TG-ROC analysis an IP ELISA protocol was derived which suggested a cut-off threshold that would allow milk testing with 97% sensitivity and specificity, respectively, relative to serum testing. Conclusions: The prevalence of N. caninum in NSW dairy cattle was higher than previously believed. When used on individual milk samples this ELISA demonstrated high sensitivity and specificity and so could be used to accurately identify N. caninum infection. TG-ROC analysis of the IP ELISA optimised the protocol and prescribed cut-off values enabling the ELISA to be used for the screening of N. caninum antibodies in the milk of dairy cattle. © 2006 Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	Veterinary Parasitology	en_US
dc.relation.isbasedon	10.1016/j.vetpar.2006.06.019	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Milk	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Cattle	en_US
dc.subject.mesh	Neospora	en_US
dc.subject.mesh	Coccidiosis	en_US
dc.subject.mesh	Cattle Diseases	en_US
dc.subject.mesh	Antibodies, Protozoan	en_US
dc.subject.mesh	Enzyme-Linked Immunosorbent Assay	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Seroepidemiologic Studies	en_US
dc.subject.mesh	ROC Curve	en_US
dc.subject.mesh	Reference Standards	en_US
dc.subject.mesh	New South Wales	en_US
dc.subject.mesh	Female	en_US
dc.title	Prevalence of Neospora caninum infection in Australian (NSW) dairy cattle estimated by a newly validated ELISA for milk	en_US
dc.type	Journal Article
utslib.citation.volume	1-2	en_US
utslib.citation.volume	142	en_US
utslib.for	070708 Veterinary Parasitology	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0704 Fisheries Sciences	en_US
utslib.for	0707 Veterinary Sciences	en_US
dc.location.activity	San Diego, CA
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	1-2	en_US
pubs.publication-status	Published	en_US
pubs.volume	142	en_US

Abstract:

Aim: To determine the performance characteristics of an Institut Pourquier (IP) enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against Neospora caninum in bovine milk and subsequent determination of the prevalence of N. caninum infection in New South Wales (NSW) dairy cattle. Methods: Matching serum and milk samples from 93 cattle were assayed in two commercially available ELISAs for the detection of anti-N. caninum antibodies. Serum test results of one ELISA (IDEXX) were used to determine the N. caninum infection status of the cattle. Optimised cut-off values for the IP ELISA using milk samples were determined by two-graph receiver operating characteristic (TG-ROC) analysis and then applied to a representative sample of 398 milk samples from dairy herds around NSW. Results: When this ELISA was applied to a representative collection of 398 milk samples from dairy cattle across NSW it demonstrated a 21.1% prevalence of N. caninum infection in those cattle. From the TG-ROC analysis an IP ELISA protocol was derived which suggested a cut-off threshold that would allow milk testing with 97% sensitivity and specificity, respectively, relative to serum testing. Conclusions: The prevalence of N. caninum in NSW dairy cattle was higher than previously believed. When used on individual milk samples this ELISA demonstrated high sensitivity and specificity and so could be used to accurately identify N. caninum infection. TG-ROC analysis of the IP ELISA optimised the protocol and prescribed cut-off values enabling the ELISA to be used for the screening of N. caninum antibodies in the milk of dairy cattle. © 2006 Elsevier B.V. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/3943