Preliminary phylogenetic identification of virulent Chlamydophila pecorum strains

Yousef Mohamad, K; Roche, SM; Myers, G; Bavoil, PM; Laroucau, K; Magnino, S; Laurent, S; Rasschaert, D; Rodolakis, A

Preliminary phylogenetic identification of virulent Chlamydophila pecorum strains

Yousef Mohamad, K Roche, SM Myers, G

Bavoil, PM Laroucau, K Magnino, S Laurent, S Rasschaert, D Rodolakis, A

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Publication Type:: Journal Article
Citation:: Infection, Genetics and Evolution, 2008, 8 (6), pp. 764 - 771
Issue Date:: 2008-12-01

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Field	Value	Language
dc.contributor.author	Yousef Mohamad, K	en_US
dc.contributor.author	Roche, SM	en_US
dc.contributor.author	Myers, G https://orcid.org/0000-0002-4756-4810	en_US
dc.contributor.author	Bavoil, PM	en_US
dc.contributor.author	Laroucau, K	en_US
dc.contributor.author	Magnino, S	en_US
dc.contributor.author	Laurent, S	en_US
dc.contributor.author	Rasschaert, D	en_US
dc.contributor.author	Rodolakis, A	en_US
dc.date.available	2008-06-19	en_US
dc.date.issued	2008-12-01	en_US
dc.identifier.citation	Infection, Genetics and Evolution, 2008, 8 (6), pp. 764 - 771	en_US
dc.identifier.issn	1567-1348	en_US
dc.identifier.uri	http://hdl.handle.net/10453/39747
dc.description.abstract	Chlamydophila pecorum is an obligate intracellular bacterium associated with different pathological conditions in ruminants, swine and koala, which is also found in the intestine of asymptomatic animals. A multi-virulence locus sequence typing (MVLST) system was developed using 19 C. pecorum strains (8 pathogenic and 11 non-pathogenic intestinal strains) isolated from ruminants of different geographical origins. To evaluate the ability of MVLST to distinguish the pathogenic from the non-pathogenic strains of C. pecorum, the sequences of 12 genes were analysed: 6 potential virulence genes (ompA, incA, incB, incC, mip and copN), 5 housekeeping genes (recA, hemD, aroC, efp, gap), and the ORF663 gene encoding a hypothetical protein (HP) that includes a variant 15-nucleotides coding tandem repeat (CTR). MVLST provided high discriminatory power (100%) in allowing to distinguish 6 of 8 pathogenic strains in a single group, and overall more discriminatory than MLST targeting housekeeping genes. ompA was the most polymorphic gene and the phylogenetic tree based only on its sequence differentiated 4 groups with high bootstrap values. The number of CTRs (rich in serine, proline and lysine) in ORF663 detected in the pathogenic strains was generally lower than that found in the intestinal strains. MVLST appears to be a promising method for the differential identification of virulent C. pecorum strains, and the ompA, incA and ORF663 genes appear to be good molecular markers for further epidemiological investigation of C. pecorum. © 2008 Elsevier B.V.	en_US
dc.relation.ispartof	Infection, Genetics and Evolution	en_US
dc.relation.isbasedon	10.1016/j.meegid.2008.06.009	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Ruminants	en_US
dc.subject.mesh	Chlamydophila	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	Bacterial Typing Techniques	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Sequence Alignment	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Tandem Repeat Sequences	en_US
dc.title	Preliminary phylogenetic identification of virulent Chlamydophila pecorum strains	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	8	en_US
utslib.for	0604 Genetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

Chlamydophila pecorum is an obligate intracellular bacterium associated with different pathological conditions in ruminants, swine and koala, which is also found in the intestine of asymptomatic animals. A multi-virulence locus sequence typing (MVLST) system was developed using 19 C. pecorum strains (8 pathogenic and 11 non-pathogenic intestinal strains) isolated from ruminants of different geographical origins. To evaluate the ability of MVLST to distinguish the pathogenic from the non-pathogenic strains of C. pecorum, the sequences of 12 genes were analysed: 6 potential virulence genes (ompA, incA, incB, incC, mip and copN), 5 housekeeping genes (recA, hemD, aroC, efp, gap), and the ORF663 gene encoding a hypothetical protein (HP) that includes a variant 15-nucleotides coding tandem repeat (CTR). MVLST provided high discriminatory power (100%) in allowing to distinguish 6 of 8 pathogenic strains in a single group, and overall more discriminatory than MLST targeting housekeeping genes. ompA was the most polymorphic gene and the phylogenetic tree based only on its sequence differentiated 4 groups with high bootstrap values. The number of CTRs (rich in serine, proline and lysine) in ORF663 detected in the pathogenic strains was generally lower than that found in the intestinal strains. MVLST appears to be a promising method for the differential identification of virulent C. pecorum strains, and the ompA, incA and ORF663 genes appear to be good molecular markers for further epidemiological investigation of C. pecorum. © 2008 Elsevier B.V.

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http://hdl.handle.net/10453/39747