High resolution typing of Chlamydophila psittaci by multilocus VNTR analysis (MLVA)

Laroucau, K; Thierry, S; Vorimore, F; Blanco, K; Kaleta, E; Hoop, R; Magnino, S; Vanrompay, D; Sachse, K; Myers, GSA; Bavoil, PM; Vergnaud, G; Pourcel, C

High resolution typing of Chlamydophila psittaci by multilocus VNTR analysis (MLVA)

Laroucau, K Thierry, S Vorimore, F Blanco, K Kaleta, E Hoop, R Magnino, S Vanrompay, D Sachse, K Myers, GSA

Bavoil, PM Vergnaud, G Pourcel, C

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Publication Type:: Journal Article
Citation:: Infection, Genetics and Evolution, 2008, 8 (2), pp. 171 - 181
Issue Date:: 2008-03-01

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Field	Value	Language
dc.contributor.author	Laroucau, K	en_US
dc.contributor.author	Thierry, S	en_US
dc.contributor.author	Vorimore, F	en_US
dc.contributor.author	Blanco, K	en_US
dc.contributor.author	Kaleta, E	en_US
dc.contributor.author	Hoop, R	en_US
dc.contributor.author	Magnino, S	en_US
dc.contributor.author	Vanrompay, D	en_US
dc.contributor.author	Sachse, K	en_US
dc.contributor.author	Myers, GSA https://orcid.org/0000-0002-4756-4810	en_US
dc.contributor.author	Bavoil, PM	en_US
dc.contributor.author	Vergnaud, G	en_US
dc.contributor.author	Pourcel, C	en_US
dc.date.available	2007-12-07	en_US
dc.date.issued	2008-03-01	en_US
dc.identifier.citation	Infection, Genetics and Evolution, 2008, 8 (2), pp. 171 - 181	en_US
dc.identifier.issn	1567-1348	en_US
dc.identifier.uri	http://hdl.handle.net/10453/39750
dc.description.abstract	A multilocus VNTR analysis (MLVA) system for detection of tandem repeats across the whole genome of Chlamydophila psittaci has been developed. Twenty selected genetic loci were initially tested on 9 avian reference strains including representatives of all major serotypes (A to F). Thereafter, 8 loci were retained for a more complete study performed on over 150 C. psittaci isolates from different bird species and geographical origins. Comparative analysis of the MLVA results and those obtained from currently available methods including serotyping and/or ompA sequencing indicate that the MLVA system provides an additional level of discrimination, with 20 distinct patterns identified to date. The newly developed MLVA system therefore provides a highly sensitive, high resolution test for the differentiation of C. psittaci isolates from different origins that is suitable for molecular epidemiological studies. © 2007 Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	Infection, Genetics and Evolution	en_US
dc.relation.isbasedon	10.1016/j.meegid.2007.12.002	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Birds	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Chlamydophila psittaci	en_US
dc.subject.mesh	Bird Diseases	en_US
dc.subject.mesh	Bacterial Typing Techniques	en_US
dc.subject.mesh	Cluster Analysis	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Minisatellite Repeats	en_US
dc.subject.mesh	Genome, Bacterial	en_US
dc.subject.mesh	Psittacosis	en_US
dc.title	High resolution typing of Chlamydophila psittaci by multilocus VNTR analysis (MLVA)	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	8	en_US
utslib.for	0604 Genetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

A multilocus VNTR analysis (MLVA) system for detection of tandem repeats across the whole genome of Chlamydophila psittaci has been developed. Twenty selected genetic loci were initially tested on 9 avian reference strains including representatives of all major serotypes (A to F). Thereafter, 8 loci were retained for a more complete study performed on over 150 C. psittaci isolates from different bird species and geographical origins. Comparative analysis of the MLVA results and those obtained from currently available methods including serotyping and/or ompA sequencing indicate that the MLVA system provides an additional level of discrimination, with 20 distinct patterns identified to date. The newly developed MLVA system therefore provides a highly sensitive, high resolution test for the differentiation of C. psittaci isolates from different origins that is suitable for molecular epidemiological studies. © 2007 Elsevier B.V. All rights reserved.

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http://hdl.handle.net/10453/39750