Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus

Myers, GSA; Parker, D; Al-Hasani, K; Kennan, RM; Seemann, T; Ren, Q; Badger, JH; Selengut, JD; DeBoy, RT; Tettelin, H; Boyce, JD; McCarl, VP; Han, X; Nelson, WC; Madupu, R; Mohamoud, Y; Holley, T; Fedorova, N; Khouri, H; Bottomley, SP; Whittington, RJ; Adler, B; Songer, JG; Rood, JI; Paulsen, IT

Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus

Parker, D Al-Hasani, K Kennan, RM Seemann, T Ren, Q Badger, JH Selengut, JD DeBoy, RT Tettelin, H Boyce, JD McCarl, VP Han, X Nelson, WC Madupu, R Mohamoud, Y Holley, T Fedorova, N Khouri, H Bottomley, SP Whittington, RJ Adler, B Songer, JG Rood, JI Paulsen, IT

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Publication Type:: Journal Article
Citation:: Nature Biotechnology, 2007, 25 (5), pp. 569 - 575
Issue Date:: 2007-05-01

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Field	Value	Language
dc.contributor.author	Myers, GSA https://orcid.org/0000-0002-4756-4810	en_US
dc.contributor.author	Parker, D	en_US
dc.contributor.author	Al-Hasani, K	en_US
dc.contributor.author	Kennan, RM	en_US
dc.contributor.author	Seemann, T	en_US
dc.contributor.author	Ren, Q	en_US
dc.contributor.author	Badger, JH	en_US
dc.contributor.author	Selengut, JD	en_US
dc.contributor.author	DeBoy, RT	en_US
dc.contributor.author	Tettelin, H	en_US
dc.contributor.author	Boyce, JD	en_US
dc.contributor.author	McCarl, VP	en_US
dc.contributor.author	Han, X	en_US
dc.contributor.author	Nelson, WC	en_US
dc.contributor.author	Madupu, R	en_US
dc.contributor.author	Mohamoud, Y	en_US
dc.contributor.author	Holley, T	en_US
dc.contributor.author	Fedorova, N	en_US
dc.contributor.author	Khouri, H	en_US
dc.contributor.author	Bottomley, SP	en_US
dc.contributor.author	Whittington, RJ	en_US
dc.contributor.author	Adler, B	en_US
dc.contributor.author	Songer, JG	en_US
dc.contributor.author	Rood, JI	en_US
dc.contributor.author	Paulsen, IT	en_US
dc.date.available	2007-04-04	en_US
dc.date.issued	2007-05-01	en_US
dc.identifier.citation	Nature Biotechnology, 2007, 25 (5), pp. 569 - 575	en_US
dc.identifier.issn	1087-0156	en_US
dc.identifier.uri	http://hdl.handle.net/10453/39754
dc.description.abstract	Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the genome is derived from lateral gene transfer. Most of these regions seem to be associated with virulence. Metabolic reconstruction indicated unsuspected capabilities, including carbohydrate utilization, electron transfer and several aerobic pathways. Global transcriptional profiling and bioinformatic analysis enabled the prediction of virulence factors and cell surface proteins. Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine. © 2007 Nature Publishing Group.	en_US
dc.relation.ispartof	Nature Biotechnology	en_US
dc.relation.isbasedon	10.1038/nbt1302	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Dichelobacter nodosus	en_US
dc.subject.mesh	Foot Rot	en_US
dc.subject.mesh	Antigens	en_US
dc.subject.mesh	Chromosome Mapping	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Genome, Bacterial	en_US
dc.title	Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	25	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	25	en_US

Abstract:

Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the genome is derived from lateral gene transfer. Most of these regions seem to be associated with virulence. Metabolic reconstruction indicated unsuspected capabilities, including carbohydrate utilization, electron transfer and several aerobic pathways. Global transcriptional profiling and bioinformatic analysis enabled the prediction of virulence factors and cell surface proteins. Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine. © 2007 Nature Publishing Group.

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http://hdl.handle.net/10453/39754