Regulation of type IV fimbrial biogenesis in Dichelobacter nodosus

Parker, D; Kennan, RM; Myers, GS; Paulsen, IT; Songer, JG; Rood, JI

Regulation of type IV fimbrial biogenesis in Dichelobacter nodosus

Parker, D Kennan, RM Myers, GS

Paulsen, IT Songer, JG Rood, JI

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Publication Type:: Journal Article
Citation:: Journal of Bacteriology, 2006, 188 (13), pp. 4801 - 4811
Issue Date:: 2006-07-01

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Field	Value	Language
dc.contributor.author	Parker, D	en_US
dc.contributor.author	Kennan, RM	en_US
dc.contributor.author	Myers, GS https://orcid.org/0000-0002-4756-4810	en_US
dc.contributor.author	Paulsen, IT	en_US
dc.contributor.author	Songer, JG	en_US
dc.contributor.author	Rood, JI	en_US
dc.date.issued	2006-07-01	en_US
dc.identifier.citation	Journal of Bacteriology, 2006, 188 (13), pp. 4801 - 4811	en_US
dc.identifier.issn	0021-9193	en_US
dc.identifier.uri	http://hdl.handle.net/10453/39759
dc.description.abstract	Type IV fimbriae are expressed by several bacterial pathogens and are essential for virulence in Dichelobacter nodosus, which causes ovine footrot. We have identified a two-component signal transduction system (PilR/S) and an alternative sigma factor (σ54) that were shown by insertional inactivation to be required for the regulation of fimbrial biogenesis in D. nodosus. Western blots showed that in both pilR and rpoN mutants, fimbrial subunit production was significantly reduced by a process that was shown to occur at a PilR- and σ54-dependent promoter. The mutants lacked surface fimbriae, which were shown to be required for the adherence of D. nodosus cells to tissue culture monolayers. The reduction in fimbrial subunit production in these mutants also resulted in a concomitant loss of the ability to secrete extracellular proteases. A maltose binding protein-PilR fusion protein was purified and was shown to bind specifically to a region located 234 to 594 bp upstream of the fimA transcriptional start point. To determine additional targets of PilR and σ54, genome-wide transcriptional profiling was performed using a whole-genome oligonucleotide microarray. The results indicated that PilR and σ54 regulated genes other fimA; these genes appear to encode surface-exposed proteins whose role in virulence is unknown. In conclusion, this study represents a significant advancement in our understanding of how the ability of D. nodosus to cause ovine footrot is regulated, as we have shown that the biogenesis of type IV fimbriae in D. nodosus is regulated by a σ54-dependent PilR/S system that also indirectly controls protease secretion. Copyright © 2006, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation.ispartof	Journal of Bacteriology	en_US
dc.relation.isbasedon	10.1128/JB.00255-06	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Fimbriae, Bacterial	en_US
dc.subject.mesh	Dichelobacter nodosus	en_US
dc.subject.mesh	Gram-Negative Bacterial Infections	en_US
dc.subject.mesh	Peptide Hydrolases	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Fimbriae Proteins	en_US
dc.subject.mesh	Transcription Factors	en_US
dc.subject.mesh	Oligonucleotide Array Sequence Analysis	en_US
dc.subject.mesh	Bacterial Adhesion	en_US
dc.subject.mesh	Signal Transduction	en_US
dc.subject.mesh	Gene Expression Regulation, Bacterial	en_US
dc.subject.mesh	Genes, Bacterial	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	RNA Polymerase Sigma 54	en_US
dc.title	Regulation of type IV fimbrial biogenesis in Dichelobacter nodosus	en_US
dc.type	Journal Article
utslib.citation.volume	13	en_US
utslib.citation.volume	188	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	13	en_US
pubs.publication-status	Published	en_US
pubs.volume	188	en_US

Abstract:

Type IV fimbriae are expressed by several bacterial pathogens and are essential for virulence in Dichelobacter nodosus, which causes ovine footrot. We have identified a two-component signal transduction system (PilR/S) and an alternative sigma factor (σ54) that were shown by insertional inactivation to be required for the regulation of fimbrial biogenesis in D. nodosus. Western blots showed that in both pilR and rpoN mutants, fimbrial subunit production was significantly reduced by a process that was shown to occur at a PilR- and σ54-dependent promoter. The mutants lacked surface fimbriae, which were shown to be required for the adherence of D. nodosus cells to tissue culture monolayers. The reduction in fimbrial subunit production in these mutants also resulted in a concomitant loss of the ability to secrete extracellular proteases. A maltose binding protein-PilR fusion protein was purified and was shown to bind specifically to a region located 234 to 594 bp upstream of the fimA transcriptional start point. To determine additional targets of PilR and σ54, genome-wide transcriptional profiling was performed using a whole-genome oligonucleotide microarray. The results indicated that PilR and σ54 regulated genes other fimA; these genes appear to encode surface-exposed proteins whose role in virulence is unknown. In conclusion, this study represents a significant advancement in our understanding of how the ability of D. nodosus to cause ovine footrot is regulated, as we have shown that the biogenesis of type IV fimbriae in D. nodosus is regulated by a σ54-dependent PilR/S system that also indirectly controls protease secretion. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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http://hdl.handle.net/10453/39759