Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5

Paulsen, IT; Press, CM; Ravel, J; Kobayashi, DY; Myers, GSA; Mavrodi, DV; DeBoy, RT; Seshadri, R; Ren, Q; Madupu, R; Dodson, RJ; Durkin, AS; Brinkac, LM; Daugherty, SC; Sullivan, SA; Rosovitz, MJ; Gwinn, ML; Zhou, L; Schneider, DJ; Cartinhour, SW; Nelson, WC; Weidman, J; Watkins, K; Tran, K; Khouri, H; Pierson, EA; Pierson, LS; Thomashow, LS; Loper, JE

Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5

Paulsen, IT Press, CM Ravel, J Kobayashi, DY Myers, GSA

Mavrodi, DV DeBoy, RT Seshadri, R Ren, Q Madupu, R Dodson, RJ Durkin, AS Brinkac, LM Daugherty, SC Sullivan, SA Rosovitz, MJ Gwinn, ML Zhou, L Schneider, DJ Cartinhour, SW Nelson, WC Weidman, J Watkins, K Tran, K Khouri, H Pierson, EA Pierson, LS Thomashow, LS Loper, JE

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Publication Type:: Journal Article
Citation:: Nature Biotechnology, 2005, 23 (7), pp. 873 - 878
Issue Date:: 2005-12-01

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Field	Value	Language
dc.contributor.author	Paulsen, IT	en_US
dc.contributor.author	Press, CM	en_US
dc.contributor.author	Ravel, J	en_US
dc.contributor.author	Kobayashi, DY	en_US
dc.contributor.author	Myers, GSA https://orcid.org/0000-0002-4756-4810	en_US
dc.contributor.author	Mavrodi, DV	en_US
dc.contributor.author	DeBoy, RT	en_US
dc.contributor.author	Seshadri, R	en_US
dc.contributor.author	Ren, Q	en_US
dc.contributor.author	Madupu, R	en_US
dc.contributor.author	Dodson, RJ	en_US
dc.contributor.author	Durkin, AS	en_US
dc.contributor.author	Brinkac, LM	en_US
dc.contributor.author	Daugherty, SC	en_US
dc.contributor.author	Sullivan, SA	en_US
dc.contributor.author	Rosovitz, MJ	en_US
dc.contributor.author	Gwinn, ML	en_US
dc.contributor.author	Zhou, L	en_US
dc.contributor.author	Schneider, DJ	en_US
dc.contributor.author	Cartinhour, SW	en_US
dc.contributor.author	Nelson, WC	en_US
dc.contributor.author	Weidman, J	en_US
dc.contributor.author	Watkins, K	en_US
dc.contributor.author	Tran, K	en_US
dc.contributor.author	Khouri, H	en_US
dc.contributor.author	Pierson, EA	en_US
dc.contributor.author	Pierson, LS	en_US
dc.contributor.author	Thomashow, LS	en_US
dc.contributor.author	Loper, JE	en_US
dc.date.available	2005-05-04	en_US
dc.date.issued	2005-12-01	en_US
dc.identifier.citation	Nature Biotechnology, 2005, 23 (7), pp. 873 - 878	en_US
dc.identifier.issn	1087-0156	en_US
dc.identifier.uri	http://hdl.handle.net/10453/39760
dc.description.abstract	Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere and produces secondary metabolites that suppress soilborne plant pathogens. The complete sequence of the 7.1-Mb Pf-5 genome was determined. We analyzed repeat sequences to identify genomic islands that, together with other approaches, suggested P. fluorescens Pf-5's recent lateral acquisitions include six secondary metabolite gene clusters, seven phage regions and a mobile genomic island. We identified various features that contribute to its commensal lifestyle on plants, including broad catabolic and transport capabilities for utilizing plant-derived compounds, the apparent ability to use a diversity of iron siderophores, detoxification systems to protect from oxidative stress, and the lack of a type III secretion system and toxins found in related pathogens. In addition to six known secondary metabolites produced by P. fluorescens Pf-5, three novel secondary metabolite biosynthesis gene clusters were also identified that may contribute to the biocontrol properties of P. fluorescens Pf-5.	en_US
dc.relation.ispartof	Nature Biotechnology	en_US
dc.relation.isbasedon	10.1038/nbt1110	en_US
dc.subject.mesh	Pseudomonas fluorescens	en_US
dc.subject.mesh	Plants	en_US
dc.subject.mesh	Siderophores	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Biological Transport	en_US
dc.subject.mesh	Genes, Bacterial	en_US
dc.subject.mesh	Genome, Bacterial	en_US
dc.subject.mesh	Multigene Family	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	23	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	23	en_US

Abstract:

Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere and produces secondary metabolites that suppress soilborne plant pathogens. The complete sequence of the 7.1-Mb Pf-5 genome was determined. We analyzed repeat sequences to identify genomic islands that, together with other approaches, suggested P. fluorescens Pf-5's recent lateral acquisitions include six secondary metabolite gene clusters, seven phage regions and a mobile genomic island. We identified various features that contribute to its commensal lifestyle on plants, including broad catabolic and transport capabilities for utilizing plant-derived compounds, the apparent ability to use a diversity of iron siderophores, detoxification systems to protect from oxidative stress, and the lack of a type III secretion system and toxins found in related pathogens. In addition to six known secondary metabolites produced by P. fluorescens Pf-5, three novel secondary metabolite biosynthesis gene clusters were also identified that may contribute to the biocontrol properties of P. fluorescens Pf-5.

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http://hdl.handle.net/10453/39760