Fibrinogen adsorption and platelet adhesion to silica surfaces with stochastic nanotopography

Lord, MS; Whitelock, JM; Simmons, A; Williams, RL; Milthorpe, BK

Fibrinogen adsorption and platelet adhesion to silica surfaces with stochastic nanotopography

Lord, MS Whitelock, JM Simmons, A Williams, RL Milthorpe, BK

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Publication Type:: Journal Article
Citation:: Biointerphases, 2014, 9 (4), pp. 041002 - ?
Issue Date:: 2014-12-01

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Field	Value	Language
dc.contributor.author	Lord, MS	en_US
dc.contributor.author	Whitelock, JM	en_US
dc.contributor.author	Simmons, A	en_US
dc.contributor.author	Williams, RL	en_US
dc.contributor.author	Milthorpe, BK https://orcid.org/0000-0002-0867-9586	en_US
dc.date.issued	2014-12-01	en_US
dc.identifier.citation	Biointerphases, 2014, 9 (4), pp. 041002 - ?	en_US
dc.identifier.uri	http://hdl.handle.net/10453/50491
dc.description.abstract	In this study, the effect of surface nanoscale roughness on fibrinogen adsorption and platelet adhesion was investigated. Nanorough silica surfaces with a low level of surface roughness (10 nm Rrms) were found to support the same level of fibrinogen adsorption as the planar silica surfaces, while nanorough silica surfaces with higher levels of surface roughness (15 nm Rrms) were found to support significantly less fibrinogen adsorption. All surfaces analyzed were found to support the same level of platelet adhesion; however, platelets were rounded in morphology on the nanorough silica surfaces while platelets were spread with a well-developed actin cytoskeleton on the planar silica. Unique quartz crystal microbalance with dissipation monitoring (QCM-D) responses was observed for the interactions between platelets and each of the surfaces. The QCM-D data indicated that platelets were more weakly attached to the nanorough silica surfaces compared with the planar silica. These data support the role of surface nanotopography in directing platelet-surface interactions even when the adsorbed fibrinogen layer is able to support the same level of platelet adhesion.	en_US
dc.relation.ispartof	Biointerphases	en_US
dc.relation.isbasedon	10.1116/1.4900993	en_US
dc.subject.mesh	Blood Platelets	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Silicon Dioxide	en_US
dc.subject.mesh	Fibrinogen	en_US
dc.subject.mesh	Cell Adhesion	en_US
dc.subject.mesh	Adsorption	en_US
dc.subject.mesh	Surface Properties	en_US
dc.title	Fibrinogen adsorption and platelet adhesion to silica surfaces with stochastic nanotopography	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	9	en_US
utslib.for	02 Physical Sciences	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

In this study, the effect of surface nanoscale roughness on fibrinogen adsorption and platelet adhesion was investigated. Nanorough silica surfaces with a low level of surface roughness (10 nm Rrms) were found to support the same level of fibrinogen adsorption as the planar silica surfaces, while nanorough silica surfaces with higher levels of surface roughness (15 nm Rrms) were found to support significantly less fibrinogen adsorption. All surfaces analyzed were found to support the same level of platelet adhesion; however, platelets were rounded in morphology on the nanorough silica surfaces while platelets were spread with a well-developed actin cytoskeleton on the planar silica. Unique quartz crystal microbalance with dissipation monitoring (QCM-D) responses was observed for the interactions between platelets and each of the surfaces. The QCM-D data indicated that platelets were more weakly attached to the nanorough silica surfaces compared with the planar silica. These data support the role of surface nanotopography in directing platelet-surface interactions even when the adsorbed fibrinogen layer is able to support the same level of platelet adhesion.

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http://hdl.handle.net/10453/50491