Isolation of two insecticidal toxins from venom of the Australian theraphosid spider Coremiocnemis tropix

Ikonomopoulou, MP; Smith, JJ; Herzig, V; Pineda, SS; Dziemborowicz, S; Er, SY; Durek, T; Gilchrist, J; Alewood, PF; Nicholson, GM; Bosmans, F; King, GF

Isolation of two insecticidal toxins from venom of the Australian theraphosid spider Coremiocnemis tropix

Ikonomopoulou, MP Smith, JJ Herzig, V Pineda, SS Dziemborowicz, S Er, SY Durek, T Gilchrist, J Alewood, PF Nicholson, GM

Bosmans, F King, GF

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Publication Type:: Journal Article
Citation:: Toxicon, 2016, 123 pp. 62 - 70
Issue Date:: 2016-12-01

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Field	Value	Language
dc.contributor.author	Ikonomopoulou, MP	en_US
dc.contributor.author	Smith, JJ	en_US
dc.contributor.author	Herzig, V	en_US
dc.contributor.author	Pineda, SS	en_US
dc.contributor.author	Dziemborowicz, S	en_US
dc.contributor.author	Er, SY	en_US
dc.contributor.author	Durek, T	en_US
dc.contributor.author	Gilchrist, J	en_US
dc.contributor.author	Alewood, PF	en_US
dc.contributor.author	Nicholson, GM https://orcid.org/0000-0002-4277-4296	en_US
dc.contributor.author	Bosmans, F	en_US
dc.contributor.author	King, GF	en_US
dc.date.available	2020-05-25T19:02:37Z
dc.date.issued	2016-12-01	en_US
dc.identifier.citation	Toxicon, 2016, 123 pp. 62 - 70	en_US
dc.identifier.issn	0041-0101	en_US
dc.identifier.uri	http://hdl.handle.net/10453/58352
dc.description.abstract	© 2016 Elsevier Ltd Sheep flystrike is caused by parasitic flies laying eggs on soiled wool or open wounds, after which the hatched maggots feed on the sheep flesh and often cause large lesions. It is a significant economic problem for the livestock industry as infestations are difficult to control due to ongoing cycles of larval development into flies followed by further egg laying. We therefore screened venom fractions from the Australian theraphosid spider Coremiocnemis tropix to identify toxins active against the sheep blowfly Lucilia cuprina, which is the primary cause of flystrike in Australia. This screen led to isolation of two insecticidal peptides, Ct1a and Ct1b, that are lethal to blowflies within 24 h of injection. The primary structure of these peptides was determined using a combination of Edman degradation and sequencing of a C. tropix venom-gland transcriptome. Ct1a and Ct1b contain 39 and 38 amino acid residues, respectively, including six cysteine residues that form three disulfide bonds. Recombinant production in bacteria (Escherichia coli) resulted in low yields of Ct1a whereas solid-phase peptide synthesis using native chemical ligation produced sufficient quantities of Ct1a for functional analyses. Synthetic Ct1a had no effect on voltage-gated sodium channels from the American cockroach Periplanata americana or the German cockroach Blattella germanica, but it was lethal to sheep blowflies with an LD50 of 1687 pmol/g.	en_US
dc.relation.ispartof	Toxicon	en_US
dc.relation.isbasedon	10.1016/j.toxicon.2016.10.013	en_US
dc.subject.classification	Toxicology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Sheep	en_US
dc.subject.mesh	Diptera	en_US
dc.subject.mesh	Spider Venoms	en_US
dc.subject.mesh	Insecticides	en_US
dc.subject.mesh	Chromatography, High Pressure Liquid	en_US
dc.subject.mesh	Sequence Alignment	en_US
dc.subject.mesh	Sequence Analysis, Protein	en_US
dc.subject.mesh	Toxicity Tests	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Mass Spectrometry	en_US
dc.subject.mesh	Transcriptome	en_US
dc.subject.mesh	Arthropod Proteins	en_US
dc.title	Isolation of two insecticidal toxins from venom of the Australian theraphosid spider Coremiocnemis tropix	en_US
dc.type	Journal Article
utslib.citation.volume	123	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	123	en_US

Abstract:

© 2016 Elsevier Ltd Sheep flystrike is caused by parasitic flies laying eggs on soiled wool or open wounds, after which the hatched maggots feed on the sheep flesh and often cause large lesions. It is a significant economic problem for the livestock industry as infestations are difficult to control due to ongoing cycles of larval development into flies followed by further egg laying. We therefore screened venom fractions from the Australian theraphosid spider Coremiocnemis tropix to identify toxins active against the sheep blowfly Lucilia cuprina, which is the primary cause of flystrike in Australia. This screen led to isolation of two insecticidal peptides, Ct1a and Ct1b, that are lethal to blowflies within 24 h of injection. The primary structure of these peptides was determined using a combination of Edman degradation and sequencing of a C. tropix venom-gland transcriptome. Ct1a and Ct1b contain 39 and 38 amino acid residues, respectively, including six cysteine residues that form three disulfide bonds. Recombinant production in bacteria (Escherichia coli) resulted in low yields of Ct1a whereas solid-phase peptide synthesis using native chemical ligation produced sufficient quantities of Ct1a for functional analyses. Synthetic Ct1a had no effect on voltage-gated sodium channels from the American cockroach Periplanata americana or the German cockroach Blattella germanica, but it was lethal to sheep blowflies with an LD50 of 1687 pmol/g.

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http://hdl.handle.net/10453/58352