Bromelain improves decrease in defecation in postoperative rats: modulation of colonic gene expression of inducible nitric oxide synthase.

Wen, S; Huang, THW; Li, GQ; Yamahara, J; Roufogalis, BD; Li, Y

Bromelain improves decrease in defecation in postoperative rats: modulation of colonic gene expression of inducible nitric oxide synthase.

Wen, S Huang, THW Li, GQ Yamahara, J Roufogalis, BD Li, Y

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Publication Type:: Journal Article
Citation:: Life Sci, 2006, 78 (9), pp. 995 - 1002
Issue Date:: 2006-01-25

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Field	Value	Language
dc.contributor.author	Wen, S	en_US
dc.contributor.author	Huang, THW	en_US
dc.contributor.author	Li, GQ	en_US
dc.contributor.author	Yamahara, J	en_US
dc.contributor.author	Roufogalis, BD	en_US
dc.contributor.author	Li, Y	en_US
dc.date.available	2005-06-06	en_US
dc.date.issued	2006-01-25	en_US
dc.identifier.citation	Life Sci, 2006, 78 (9), pp. 995 - 1002	en_US
dc.identifier.issn	0024-3205	en_US
dc.identifier.uri	http://hdl.handle.net/10453/818
dc.description.abstract	Ileus continues to be a common consequence of abdominal surgery, causing significant patient discomfort and often leading to more serious problems. The therapy available is limited, hence, ileus remains an important clinical problem. Activation of inducible nitric oxide synthase (iNOS) directly modulates intestinal dysmotility after bowel manipulation and plays an essential role in initiating intestinal inflammation. Nuclear factor (NF)-kappaB is known to be a critical component of iNOS gene transcriptional activation in response to inflammatory stimuli. Bromelain is a crude extract from the pineapple stem, which is sold as a nutritional supplement to "promote digestive health" and as an anti-inflammatory medication in some developed countries. Here, we have found that oral administration of bromelain improves decrease in defecation in abdominal postoperative rats. Results showed that bromelain increased the wet weight, dry weight, water content and number of fecal pellets in laparotomized plus mechanically manipulated rats, suggesting improvement of postoperative ileus. Furthermore, bromelain treatment inhibited overexpressed iNOS mRNA and restored down-regulated inhibitor kappaBalpha mRNA in the colon of the postoperative rats. From the in vitro experiments, bromelain inhibits lipopolysaccharide (LPS)-induced nitrite overproduction in macrophage cell lines and LPS-induced NF-kappaB luciferase reporter gene expression in RAW264.7 macrophages transfected with NF-kappaB luciferase reporter gene. Thus, our findings suggest that bromelain improves decrease in defecation in postoperative rats, at least in part, by inhibiting colonic iNOS overexpression via NF-kappaB pathway. Our data indicates that bromelain may benefit patients with postoperative ileus.	en_US
dc.language	eng	en_US
dc.relation.ispartof	Life Sci	en_US
dc.relation.isbasedon	10.1016/j.lfs.2005.06.007	en_US
dc.subject.classification	Pharmacology & Pharmacy	en_US
dc.subject.mesh	Colon	en_US
dc.subject.mesh	Macrophages	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Rats	en_US
dc.subject.mesh	Nitrites	en_US
dc.subject.mesh	Neostigmine	en_US
dc.subject.mesh	Serotonin	en_US
dc.subject.mesh	Bromelains	en_US
dc.subject.mesh	Luciferases	en_US
dc.subject.mesh	Lipopolysaccharides	en_US
dc.subject.mesh	NF-kappa B	en_US
dc.subject.mesh	Cholinesterase Inhibitors	en_US
dc.subject.mesh	Anti-Inflammatory Agents, Non-Steroidal	en_US
dc.subject.mesh	Postoperative Period	en_US
dc.subject.mesh	Tissue Culture Techniques	en_US
dc.subject.mesh	Transfection	en_US
dc.subject.mesh	Gene Expression Regulation, Enzymologic	en_US
dc.subject.mesh	Defecation	en_US
dc.subject.mesh	Gastrointestinal Motility	en_US
dc.subject.mesh	Nitric Oxide Synthase Type II	en_US
dc.title	Bromelain improves decrease in defecation in postoperative rats: modulation of colonic gene expression of inducible nitric oxide synthase.	en_US
dc.type	Journal Article
utslib.citation.volume	9	en_US
utslib.citation.volume	78	en_US
utslib.location.activity	Netherlands	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	9	en_US
pubs.publication-status	Published	en_US
pubs.volume	78	en_US

Abstract:

Ileus continues to be a common consequence of abdominal surgery, causing significant patient discomfort and often leading to more serious problems. The therapy available is limited, hence, ileus remains an important clinical problem. Activation of inducible nitric oxide synthase (iNOS) directly modulates intestinal dysmotility after bowel manipulation and plays an essential role in initiating intestinal inflammation. Nuclear factor (NF)-kappaB is known to be a critical component of iNOS gene transcriptional activation in response to inflammatory stimuli. Bromelain is a crude extract from the pineapple stem, which is sold as a nutritional supplement to "promote digestive health" and as an anti-inflammatory medication in some developed countries. Here, we have found that oral administration of bromelain improves decrease in defecation in abdominal postoperative rats. Results showed that bromelain increased the wet weight, dry weight, water content and number of fecal pellets in laparotomized plus mechanically manipulated rats, suggesting improvement of postoperative ileus. Furthermore, bromelain treatment inhibited overexpressed iNOS mRNA and restored down-regulated inhibitor kappaBalpha mRNA in the colon of the postoperative rats. From the in vitro experiments, bromelain inhibits lipopolysaccharide (LPS)-induced nitrite overproduction in macrophage cell lines and LPS-induced NF-kappaB luciferase reporter gene expression in RAW264.7 macrophages transfected with NF-kappaB luciferase reporter gene. Thus, our findings suggest that bromelain improves decrease in defecation in postoperative rats, at least in part, by inhibiting colonic iNOS overexpression via NF-kappaB pathway. Our data indicates that bromelain may benefit patients with postoperative ileus.

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http://hdl.handle.net/10453/818