Identification of membrane-associated proteins from Campylobacter jejuni strains using complementary proteomics technologies

Cordwell, SJ; Len, ACL; Touma, RG; Scott, NE; Falconer, L; Jones, D; Connolly, A; Crossett, B; Djordjevic, SP

Identification of membrane-associated proteins from Campylobacter jejuni strains using complementary proteomics technologies

Cordwell, SJ Len, ACL Touma, RG Scott, NE Falconer, L Jones, D Connolly, A Crossett, B Djordjevic, SP

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Publication Type:: Journal Article
Citation:: Proteomics, 2008, 8 (1), pp. 122 - 139
Issue Date:: 2008-01-01

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Field	Value	Language
dc.contributor.author	Cordwell, SJ	en_US
dc.contributor.author	Len, ACL	en_US
dc.contributor.author	Touma, RG	en_US
dc.contributor.author	Scott, NE	en_US
dc.contributor.author	Falconer, L	en_US
dc.contributor.author	Jones, D	en_US
dc.contributor.author	Connolly, A	en_US
dc.contributor.author	Crossett, B	en_US
dc.contributor.author	Djordjevic, SP https://orcid.org/0000-0001-9301-5372	en_US
dc.date.issued	2008-01-01	en_US
dc.identifier.citation	Proteomics, 2008, 8 (1), pp. 122 - 139	en_US
dc.identifier.issn	1615-9853	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8713
dc.description.abstract	Campylobacter jejuni is the leading cause of food- and water-borne illness world-wide. The membrane-associated proteome of a recent C. jejuni gastrointestinal isolate (JHH1) was generated by sodium carbonate precipitation and ultracentrifugation followed by 2-DE and MALDI-TOF MS as well as 2-DLC (strong cation exchange followed by RP chromatography) of trypsin digests coupled to MS/MS (2-DLC/MS/MS). 2-DE/MS identified 77 proteins, 44 of which were predicted membrane proteins, while 2-DLC/MS/MS identified 432 proteins, of which 206 were predicted to be membrane associated. Atotal of 453 unique proteins (27.4% ofthe C. jejuni theoretical proteome), including 187 bona fide membrane proteins were identified in this study. Membrane proteins were also compared between C. jejuni JHH1 and ATCC 700297 to identify factors potentially associated with increased gastrointestinal virulence. We identified 28 proteins that were significantly (>two-fold) more abundant in, or unique to, JHH1, including eight proteins involved in chemotaxis signal transduction and flagellar motility, the amino acid-binding surface antigens CjaA and CjaC, and four outer membrane proteins (OMPs) of unknown function (Cj0129c, Cj1031, Cj1279c, and Cj1721c). Immunoblotting using convalescent patient sera generated postgastrointestinal infection revealed 13 (JHH1) and 12 (ATCC 700297) immunoreactive proteins. These included flagellin (FlaA) and CadF as well as Omp18, Omp50, Cj1721c, PEB1A, PEB2, and PEB4A. This study provides a comprehensive analysis of membrane-associated proteins from C. jejuni. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.	en_US
dc.relation.ispartof	Proteomics	en_US
dc.relation.isbasedon	10.1002/pmic.200700561	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Campylobacter jejuni	en_US
dc.subject.mesh	Campylobacter Infections	en_US
dc.subject.mesh	Bacterial Outer Membrane Proteins	en_US
dc.subject.mesh	Chromatography, Liquid	en_US
dc.subject.mesh	Combinatorial Chemistry Techniques	en_US
dc.subject.mesh	Electrophoresis, Gel, Two-Dimensional	en_US
dc.subject.mesh	Proteomics	en_US
dc.subject.mesh	Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization	en_US
dc.subject.mesh	Tandem Mass Spectrometry	en_US
dc.title	Identification of membrane-associated proteins from Campylobacter jejuni strains using complementary proteomics technologies	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	8	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	08 Information and Computing Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

Campylobacter jejuni is the leading cause of food- and water-borne illness world-wide. The membrane-associated proteome of a recent C. jejuni gastrointestinal isolate (JHH1) was generated by sodium carbonate precipitation and ultracentrifugation followed by 2-DE and MALDI-TOF MS as well as 2-DLC (strong cation exchange followed by RP chromatography) of trypsin digests coupled to MS/MS (2-DLC/MS/MS). 2-DE/MS identified 77 proteins, 44 of which were predicted membrane proteins, while 2-DLC/MS/MS identified 432 proteins, of which 206 were predicted to be membrane associated. Atotal of 453 unique proteins (27.4% ofthe C. jejuni theoretical proteome), including 187 bona fide membrane proteins were identified in this study. Membrane proteins were also compared between C. jejuni JHH1 and ATCC 700297 to identify factors potentially associated with increased gastrointestinal virulence. We identified 28 proteins that were significantly (>two-fold) more abundant in, or unique to, JHH1, including eight proteins involved in chemotaxis signal transduction and flagellar motility, the amino acid-binding surface antigens CjaA and CjaC, and four outer membrane proteins (OMPs) of unknown function (Cj0129c, Cj1031, Cj1279c, and Cj1721c). Immunoblotting using convalescent patient sera generated postgastrointestinal infection revealed 13 (JHH1) and 12 (ATCC 700297) immunoreactive proteins. These included flagellin (FlaA) and CadF as well as Omp18, Omp50, Cj1721c, PEB1A, PEB2, and PEB4A. This study provides a comprehensive analysis of membrane-associated proteins from C. jejuni. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

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http://hdl.handle.net/10453/8713