Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae

Trussart, M; Yus, E; Martinez, S; Baù, D; Tahara, YO; Pengo, T; Widjaja, M; Kretschmer, S; Swoger, J; Djordjevic, S; Turnbull, L; Whitchurch, C; Miyata, M; Marti-Renom, MA; Lluch-Senar, M; Serrano, L

Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae

Trussart, M Yus, E Martinez, S Baù, D Tahara, YO Pengo, T Widjaja, M

Kretschmer, S Swoger, J Djordjevic, S

Turnbull, L

Whitchurch, C

Miyata, M Marti-Renom, MA Lluch-Senar, M Serrano, L

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Publication Type:: Journal Article
Citation:: Nature Communications, 2017, 8
Issue Date:: 2017-03-08

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Field	Value	Language
dc.contributor.author	Trussart, M	en_US
dc.contributor.author	Yus, E	en_US
dc.contributor.author	Martinez, S	en_US
dc.contributor.author	Baù, D	en_US
dc.contributor.author	Tahara, YO	en_US
dc.contributor.author	Pengo, T	en_US
dc.contributor.author	Widjaja, M https://orcid.org/0000-0003-1509-0443	en_US
dc.contributor.author	Kretschmer, S	en_US
dc.contributor.author	Swoger, J	en_US
dc.contributor.author	Djordjevic, S https://orcid.org/0000-0001-9301-5372	en_US
dc.contributor.author	Turnbull, L https://orcid.org/0000-0002-9255-9033	en_US
dc.contributor.author	Whitchurch, C https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Miyata, M	en_US
dc.contributor.author	Marti-Renom, MA	en_US
dc.contributor.author	Lluch-Senar, M	en_US
dc.contributor.author	Serrano, L	en_US
dc.date.available	2017-01-20	en_US
dc.date.issued	2017-03-08	en_US
dc.identifier.citation	Nature Communications, 2017, 8	en_US
dc.identifier.uri	http://hdl.handle.net/10453/87279
dc.description.abstract	© 2017 The Author(s). DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine the structure of the Mycoplasma pneumoniae chromosome at a 10 kb resolution. We find a defined structure, with a global symmetry between two arms that connect opposite poles, one bearing the chromosomal Ori and the other the midpoint. Analysis of local structures at a 3 kb resolution indicates that the chromosome is organized into domains ranging from 15 to 33 kb. We provide evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization. This study extends the current understanding of bacterial genome organization and demonstrates that a defined chromosomal structure is a universal feature of living systems.	en_US
dc.relation.ispartof	Nature Communications	en_US
dc.relation.isbasedon	10.1038/ncomms14665	en_US
dc.subject.mesh	Chromosomes, Bacterial	en_US
dc.subject.mesh	Chromosome Structures	en_US
dc.subject.mesh	Mycoplasma pneumoniae	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	DNA, Superhelical	en_US
dc.subject.mesh	Microscopy	en_US
dc.subject.mesh	Gene Expression Regulation, Bacterial	en_US
dc.subject.mesh	Molecular Conformation	en_US
dc.subject.mesh	Nucleic Acid Conformation	en_US
dc.subject.mesh	Genome, Bacterial	en_US
dc.title	Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

© 2017 The Author(s). DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine the structure of the Mycoplasma pneumoniae chromosome at a 10 kb resolution. We find a defined structure, with a global symmetry between two arms that connect opposite poles, one bearing the chromosomal Ori and the other the midpoint. Analysis of local structures at a 3 kb resolution indicates that the chromosome is organized into domains ranging from 15 to 33 kb. We provide evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization. This study extends the current understanding of bacterial genome organization and demonstrates that a defined chromosomal structure is a universal feature of living systems.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/87279