Chlorophyll f distribution and dynamics in cyanobacterial beachrock biofilms

Trampe, E; Kühl, M

Chlorophyll f distribution and dynamics in cyanobacterial beachrock biofilms

Trampe, E Kühl, M

Permalink

Publication Type:: Journal Article
Citation:: Journal of Phycology, 2016, 52 (6), pp. 990 - 996
Issue Date:: 2016-12-01

Closed Access

	Filename	Description	Size
	Trampe_et_al-2016-Journal_of_Phycology.pdf	Published Version	1.09 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Trampe, E	en_US
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.date.available	2016-07-03	en_US
dc.date.issued	2016-12-01	en_US
dc.identifier.citation	Journal of Phycology, 2016, 52 (6), pp. 990 - 996	en_US
dc.identifier.issn	0022-3646	en_US
dc.identifier.uri	http://hdl.handle.net/10453/90532
dc.description.abstract	© 2016 Phycological Society of America Chlorophyll (Chl) f, the most far-red (720–740 nm) absorbing Chl species, was discovered in cyanobacterial isolates from stromatolites and subsequently in other habitats as well. However, the spatial distribution and temporal dynamics of Chl f in a natural habitat have so far not been documented. Here, we report the presence of Chl f in cyanobacterial beachrock biofilms. Hyperspectral imaging on cross-sections of beachrock from Heron Island (Great Barrier Reef, Australia), showed a strong and widely distributed signature of Chl f absorption in an endolithic layer below the dense cyanobacterial surface biofilm that could be localized to aggregates of Chroococcidiopsis-like unicellular cyanobacteria packed within a thick common sheath. High-pressure liquid chromatography-based pigment analyses showed in situ ratios of Chl f to Chl a of 5% in brown-pigmented zones of the beachrock, with lower ratios of ~0.5% in the black- and pink-pigmented biofilm zones. Enrichment experiments with black beachrock biofilm showed stimulated synthesis of Chl f and Chl d when grown under near-infrared radiation (NIR; 740 nm), with a Chl f to Chl a ratio increasing 4-fold to 2%, whereas the Chl d to Chl a ratio went from 0% to 0.8%. Enrichments grown under white light (400–700 nm) produced no detectable amounts of either Chl d or Chl f. Beachrock cyanobacteria thus exhibited characteristics of far-red light photoacclimation, enabling Chl f -containing cyanobacteria to thrive in optical niches deprived of visible light when sufficient NIR is prevalent.	en_US
dc.relation.ispartof	Journal of Phycology	en_US
dc.relation.isbasedon	10.1111/jpy.12450	en_US
dc.subject.classification	Marine Biology & Hydrobiology	en_US
dc.subject.mesh	Biofilms	en_US
dc.subject.mesh	Cyanobacteria	en_US
dc.subject.mesh	Chlorophyll	en_US
dc.subject.mesh	Queensland	en_US
dc.subject.mesh	Coral Reefs	en_US
dc.title	Chlorophyll f distribution and dynamics in cyanobacterial beachrock biofilms	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	52	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0704 Fisheries Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	52	en_US

Abstract:

© 2016 Phycological Society of America Chlorophyll (Chl) f, the most far-red (720–740 nm) absorbing Chl species, was discovered in cyanobacterial isolates from stromatolites and subsequently in other habitats as well. However, the spatial distribution and temporal dynamics of Chl f in a natural habitat have so far not been documented. Here, we report the presence of Chl f in cyanobacterial beachrock biofilms. Hyperspectral imaging on cross-sections of beachrock from Heron Island (Great Barrier Reef, Australia), showed a strong and widely distributed signature of Chl f absorption in an endolithic layer below the dense cyanobacterial surface biofilm that could be localized to aggregates of Chroococcidiopsis-like unicellular cyanobacteria packed within a thick common sheath. High-pressure liquid chromatography-based pigment analyses showed in situ ratios of Chl f to Chl a of 5% in brown-pigmented zones of the beachrock, with lower ratios of ~0.5% in the black- and pink-pigmented biofilm zones. Enrichment experiments with black beachrock biofilm showed stimulated synthesis of Chl f and Chl d when grown under near-infrared radiation (NIR; 740 nm), with a Chl f to Chl a ratio increasing 4-fold to 2%, whereas the Chl d to Chl a ratio went from 0% to 0.8%. Enrichments grown under white light (400–700 nm) produced no detectable amounts of either Chl d or Chl f. Beachrock cyanobacteria thus exhibited characteristics of far-red light photoacclimation, enabling Chl f -containing cyanobacteria to thrive in optical niches deprived of visible light when sufficient NIR is prevalent.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/90532