Development of a toxicity identification evaluation protocol using chlorophyll-a fluorescence in a marine microalga

Strom, D; Ralph, PJ; Stauber, JL

Development of a toxicity identification evaluation protocol using chlorophyll-a fluorescence in a marine microalga

Strom, D Ralph, PJ

Stauber, JL

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Publication Type:: Journal Article
Citation:: Archives of Environmental Contamination and Toxicology, 2009, 56 (1), pp. 30 - 38
Issue Date:: 2009-01-01

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Field	Value	Language
dc.contributor.author	Strom, D	en_US
dc.contributor.author	Ralph, PJ https://orcid.org/0000-0002-3103-7346	en_US
dc.contributor.author	Stauber, JL	en_US
dc.date.available	2008-03-31	en_US
dc.date.issued	2009-01-01	en_US
dc.identifier.citation	Archives of Environmental Contamination and Toxicology, 2009, 56 (1), pp. 30 - 38	en_US
dc.identifier.issn	0090-4341	en_US
dc.identifier.uri	http://hdl.handle.net/10453/9602
dc.description.abstract	Growth inhibition bioassays with the microalga Nitzschia closterium have recently been applied in marine Toxicity Identification Evaluation (TIE) testing. However, the 48-h test duration can result in substantial loss of toxicants over time, which might lead to an underestimation of the sample toxicity. Although shorter-term microalgal bioassays can minimize such losses, there are few bioassays available and none are adapted for marine TIE testing. The acute (5-min) chlorophyll-a fluorescence bioassay is one alternative; however, this bioassay was developed for detecting herbicides in freshwater aquatic systems and its suitability for marine TIE testing was not known. In this study, a chlorophyll-a fluorescence bioassay using the marine microalga Isochrysis galbana was able to detect contaminants other than herbicides at environmentally relevant concentrations and tolerated the physical and chemical manipulations needed for a Phase I TIE. Phase I TIE procedures were successfully developed using this chlorophyll-a fluorescence bioassay and used to identify all classes of contaminants present in a synthetic mixture of known chemical composition. In addition, TIEs with both the acute fluorescence bioassay and the standard growth inhibition bioassay identified the same classes of toxicants in a sample of an unknown complex effluent. Even though the acute chlorophyll-a fluorescence end point was less sensitive than the chronic cell division end point, TIEs with the chlorophyll-a fluorescence bioassay provided a rapid and attractive alternative to longer-duration bioassays. © 2008 Springer Science+Business Media, LLC.	en_US
dc.relation.ispartof	Archives of Environmental Contamination and Toxicology	en_US
dc.relation.isbasedon	10.1007/s00244-008-9174-x	en_US
dc.subject.classification	Toxicology	en_US
dc.subject.mesh	Diatoms	en_US
dc.subject.mesh	Chlorophyll	en_US
dc.subject.mesh	Xenobiotics	en_US
dc.subject.mesh	Water Pollutants, Chemical	en_US
dc.subject.mesh	Biological Assay	en_US
dc.subject.mesh	Environmental Monitoring	en_US
dc.subject.mesh	Dose-Response Relationship, Drug	en_US
dc.subject.mesh	Fluorescence	en_US
dc.subject.mesh	Toxicity Tests, Acute	en_US
dc.title	Development of a toxicity identification evaluation protocol using chlorophyll-a fluorescence in a marine microalga	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	56	en_US
utslib.for	0699 Other Biological Sciences	en_US
dc.location.activity	ISI:000261938500004	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	56	en_US

Abstract:

Growth inhibition bioassays with the microalga Nitzschia closterium have recently been applied in marine Toxicity Identification Evaluation (TIE) testing. However, the 48-h test duration can result in substantial loss of toxicants over time, which might lead to an underestimation of the sample toxicity. Although shorter-term microalgal bioassays can minimize such losses, there are few bioassays available and none are adapted for marine TIE testing. The acute (5-min) chlorophyll-a fluorescence bioassay is one alternative; however, this bioassay was developed for detecting herbicides in freshwater aquatic systems and its suitability for marine TIE testing was not known. In this study, a chlorophyll-a fluorescence bioassay using the marine microalga Isochrysis galbana was able to detect contaminants other than herbicides at environmentally relevant concentrations and tolerated the physical and chemical manipulations needed for a Phase I TIE. Phase I TIE procedures were successfully developed using this chlorophyll-a fluorescence bioassay and used to identify all classes of contaminants present in a synthetic mixture of known chemical composition. In addition, TIEs with both the acute fluorescence bioassay and the standard growth inhibition bioassay identified the same classes of toxicants in a sample of an unknown complex effluent. Even though the acute chlorophyll-a fluorescence end point was less sensitive than the chronic cell division end point, TIEs with the chlorophyll-a fluorescence bioassay provided a rapid and attractive alternative to longer-duration bioassays. © 2008 Springer Science+Business Media, LLC.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/9602