Phylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative shiga toxin 1 and 2 prophages

Mellor, GE; Sim, EM; Barlow, RS; D'Astek, BA; Galli, L; Chinen, I; Rivas, M; Gobius, KS

Phylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative shiga toxin 1 and 2 prophages

Mellor, GE Sim, EM

Barlow, RS D'Astek, BA Galli, L Chinen, I Rivas, M Gobius, KS

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Publication Type:: Journal Article
Citation:: Applied and Environmental Microbiology, 2012, 78 (13), pp. 4724 - 4731
Issue Date:: 2012-07-01

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Field	Value	Language
dc.contributor.author	Mellor, GE	en_US
dc.contributor.author	Sim, EM https://orcid.org/0000-0002-3716-7344	en_US
dc.contributor.author	Barlow, RS	en_US
dc.contributor.author	D'Astek, BA	en_US
dc.contributor.author	Galli, L	en_US
dc.contributor.author	Chinen, I	en_US
dc.contributor.author	Rivas, M	en_US
dc.contributor.author	Gobius, KS	en_US
dc.date.issued	2012-07-01	en_US
dc.identifier.citation	Applied and Environmental Microbiology, 2012, 78 (13), pp. 4724 - 4731	en_US
dc.identifier.issn	0099-2240	en_US
dc.identifier.uri	http://hdl.handle.net/10453/113617
dc.description.abstract	Shiga toxigenic Escherichia coli O157 is the leading cause of hemolytic uremic syndrome (HUS) worldwide. The frequencies of stx genotypes and the incidences of O157-related illness and HUS vary significantly between Argentina and Australia. Locusspecific polymorphism analysis revealed that lineage I/II (LI/II) E. coli O157 isolates were most prevalent in Argentina (90%) and Australia (88%). Argentinean LI/II isolates were shown to belong to clades 4 (28%) and 8 (72%), while Australian LI/II isolates were identified as clades 6 (15%), 7 (83%), and 8 (2%). Clade 8 was significantly associated with Shiga toxin bacteriophage insertion (SBI) type stx2 (locus of insertion, argW) in Argentinean isolates (P<0.0001). In Argentinean LI/II strains, stx2 is carried by a prophage inserted at argW, whereas in Australian LI/II strains the argW locus is occupied by the novel stx1 prophage. In both Argentinean and Australian LI/II strains, stx2c is almost exclusively carried by a prophage inserted at sbcB. However, alternative q933- or q21-related alleles were identified in the Australian stx2c prophage. Argentinean LI/II isolates were also distinguished from Australian isolates by the presence of the putative virulence determinant ECSP_3286 and the predominance of motile O157:H7 strains. Characteristics common to both Argentinean and Australian LI/II O157 strains included the presence of putative virulence determinants (ECSP_3620, ECSP_0242, ECSP_2687, ECSP_2870, and ECSP_2872) and the predominance of the tir255T allele. These data support further understanding of O157 phylogeny and may foster greater insight into the differential virulence of O157 lineages. © 2012, American Society for Microbiology.	en_US
dc.relation.ispartof	Applied and Environmental Microbiology	en_US
dc.relation.isbasedon	10.1128/AEM.00365-12	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Escherichia coli O157	en_US
dc.subject.mesh	Coliphages	en_US
dc.subject.mesh	Prophages	en_US
dc.subject.mesh	Escherichia coli Infections	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	Virulence Factors	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Argentina	en_US
dc.subject.mesh	Australia	en_US
dc.subject.mesh	Shiga Toxin 1	en_US
dc.subject.mesh	Shiga Toxin 2	en_US
dc.title	Phylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative shiga toxin 1 and 2 prophages	en_US
dc.type	Journal Article
utslib.citation.volume	13	en_US
utslib.citation.volume	78	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	13	en_US
pubs.publication-status	Published	en_US
pubs.volume	78	en_US

Abstract:

Shiga toxigenic Escherichia coli O157 is the leading cause of hemolytic uremic syndrome (HUS) worldwide. The frequencies of stx genotypes and the incidences of O157-related illness and HUS vary significantly between Argentina and Australia. Locusspecific polymorphism analysis revealed that lineage I/II (LI/II) E. coli O157 isolates were most prevalent in Argentina (90%) and Australia (88%). Argentinean LI/II isolates were shown to belong to clades 4 (28%) and 8 (72%), while Australian LI/II isolates were identified as clades 6 (15%), 7 (83%), and 8 (2%). Clade 8 was significantly associated with Shiga toxin bacteriophage insertion (SBI) type stx2 (locus of insertion, argW) in Argentinean isolates (P<0.0001). In Argentinean LI/II strains, stx2 is carried by a prophage inserted at argW, whereas in Australian LI/II strains the argW locus is occupied by the novel stx1 prophage. In both Argentinean and Australian LI/II strains, stx2c is almost exclusively carried by a prophage inserted at sbcB. However, alternative q933- or q21-related alleles were identified in the Australian stx2c prophage. Argentinean LI/II isolates were also distinguished from Australian isolates by the presence of the putative virulence determinant ECSP_3286 and the predominance of motile O157:H7 strains. Characteristics common to both Argentinean and Australian LI/II O157 strains included the presence of putative virulence determinants (ECSP_3620, ECSP_0242, ECSP_2687, ECSP_2870, and ECSP_2872) and the predominance of the tir255T allele. These data support further understanding of O157 phylogeny and may foster greater insight into the differential virulence of O157 lineages. © 2012, American Society for Microbiology.

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http://hdl.handle.net/10453/113617