Human papillomavirus 16 E6 modulates the expression of miR-496 in oropharyngeal cancer

Mason, D; Zhang, X; Marques, TM; Rose, B; Khoury, S; Hill, M; Deutsch, F; Lyons, JG; Gama-Carvalho, M; Tran, N

Human papillomavirus 16 E6 modulates the expression of miR-496 in oropharyngeal cancer

Mason, D Zhang, X Marques, TM Rose, B Khoury, S

Hill, M

Deutsch, F Lyons, JG Gama-Carvalho, M Tran, N

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Publication Type:: Journal Article
Citation:: Virology, 2018, 521 pp. 149 - 157
Issue Date:: 2018-08-01

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Field	Value	Language
dc.contributor.author	Mason, D	en_US
dc.contributor.author	Zhang, X	en_US
dc.contributor.author	Marques, TM	en_US
dc.contributor.author	Rose, B	en_US
dc.contributor.author	Khoury, S https://orcid.org/0000-0001-5631-7478	en_US
dc.contributor.author	Hill, M https://orcid.org/0000-0003-4637-1483	en_US
dc.contributor.author	Deutsch, F	en_US
dc.contributor.author	Lyons, JG	en_US
dc.contributor.author	Gama-Carvalho, M	en_US
dc.contributor.author	Tran, N https://orcid.org/0000-0001-7747-2530	en_US
dc.date.available	2018-05-28	en_US
dc.date.issued	2018-08-01	en_US
dc.identifier.citation	Virology, 2018, 521 pp. 149 - 157	en_US
dc.identifier.issn	0042-6822	en_US
dc.identifier.uri	http://hdl.handle.net/10453/126190
dc.description.abstract	© 2018 Human papillomavirus (HPV), notably type 16, is a risk factor for up to 75% of oropharyngeal squamous cell carcinomas (SCC). It has been demonstrated that small non-coding RNAs known as microRNAs play a vital role in the cellular transformation process. In this study, we used an LNA array to further investigate the impact of HPV16 on the expression of microRNAs in oropharyngeal (tonsillar) cancer. A number of miRNAs were found to be deregulated, with miR-496 showing a four-fold decrease. Over-expression of the high risk E6 oncoprotein down-regulated miR-496, impacting upon the post-transcriptional control of the transcription factor E2F2. These HPV specific miRNAs were integrated with the HPV16 interactome to identify possible mechanistic pathways. These analyses provide insights into novel molecular interactions between HPV16 and miRNAs in oropharyngeal cancers.	en_US
dc.relation.ispartof	Virology	en_US
dc.relation.isbasedon	10.1016/j.virol.2018.05.022	en_US
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Virology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Carcinoma, Squamous Cell	en_US
dc.subject.mesh	Oropharyngeal Neoplasms	en_US
dc.subject.mesh	Oncogene Proteins, Viral	en_US
dc.subject.mesh	Repressor Proteins	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	Down-Regulation	en_US
dc.subject.mesh	E2F2 Transcription Factor	en_US
dc.subject.mesh	Human papillomavirus 16	en_US
dc.subject.mesh	Gene Regulatory Networks	en_US
dc.subject.mesh	Host-Pathogen Interactions	en_US
dc.title	Human papillomavirus 16 E6 modulates the expression of miR-496 in oropharyngeal cancer	en_US
dc.type	Journal Article
utslib.citation.volume	521	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	1101 Medical Biochemistry and Metabolomics	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access	*
pubs.publication-status	Published	en_US
pubs.volume	521	en_US

Abstract:

© 2018 Human papillomavirus (HPV), notably type 16, is a risk factor for up to 75% of oropharyngeal squamous cell carcinomas (SCC). It has been demonstrated that small non-coding RNAs known as microRNAs play a vital role in the cellular transformation process. In this study, we used an LNA array to further investigate the impact of HPV16 on the expression of microRNAs in oropharyngeal (tonsillar) cancer. A number of miRNAs were found to be deregulated, with miR-496 showing a four-fold decrease. Over-expression of the high risk E6 oncoprotein down-regulated miR-496, impacting upon the post-transcriptional control of the transcription factor E2F2. These HPV specific miRNAs were integrated with the HPV16 interactome to identify possible mechanistic pathways. These analyses provide insights into novel molecular interactions between HPV16 and miRNAs in oropharyngeal cancers.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/126190