Static droplet array for culturing single live adherent cells in an isolated chemical microenvironment

Hassanzadeh-Barforoushi, A; Law, AMK; Hejri, A; Asadnia, M; Ormandy, CJ; Gallego-Ortega, D; Ebrahimi Warkiani, M

Static droplet array for culturing single live adherent cells in an isolated chemical microenvironment

Hassanzadeh-Barforoushi, A Law, AMK Hejri, A Asadnia, M Ormandy, CJ Gallego-Ortega, D Ebrahimi Warkiani, M

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Publication Type:: Journal Article
Citation:: Lab on a Chip, 2018, 18 (15), pp. 2156 - 2166
Issue Date:: 2018-01-01

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Field	Value	Language
dc.contributor.author	Hassanzadeh-Barforoushi, A	en_US
dc.contributor.author	Law, AMK	en_US
dc.contributor.author	Hejri, A	en_US
dc.contributor.author	Asadnia, M	en_US
dc.contributor.author	Ormandy, CJ	en_US
dc.contributor.author	Gallego-Ortega, D	en_US
dc.contributor.author	Ebrahimi Warkiani, M https://orcid.org/0000-0002-4184-1944	en_US
dc.date.issued	2018-01-01	en_US
dc.identifier.citation	Lab on a Chip, 2018, 18 (15), pp. 2156 - 2166	en_US
dc.identifier.issn	1473-0197	en_US
dc.identifier.uri	http://hdl.handle.net/10453/132023
dc.description.abstract	© 2018 The Royal Society of Chemistry. We present here a new method to easily and reliably generate an array of hundreds of dispersed nanoliter-volume semi-droplets for single-cells culture and analysis. The liquid segmentation step occurs directly in indexed traps by a tweezer-like mechanism and is stabilized by spatial confinement. Unlike common droplet-based techniques, the semi-droplet wets its surrounding trap walls thus supporting the culturing of both adherent and non-adherent cells. To eliminate cross-droplet cell migration and chemical cross-talk each semi-droplet is separated from a nearby trap by an ∼80 pL air plug. The overall setup and injection procedure takes less than 10 minutes, is insensitive to fabrication defects and supports cell recovery for downstream analysis. The method offers a new approach to easily capture, image and culture single cells in a chemically isolated microenvironment as a preliminary step towards high-throughput single-cell assays.	en_US
dc.relation	http://purl.org/au-research/grants/arc/DP170103704
dc.relation	http://purl.org/au-research/grants/arc/DP180103003
dc.relation	http://purl.org/au-research/grants/nhmrc/GNT1143377
dc.relation.ispartof	Lab on a Chip	en_US
dc.relation.isbasedon	10.1039/c8lc00403j	en_US
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Cell Line, Tumor	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Cell Culture Techniques	en_US
dc.subject.mesh	Equipment Design	en_US
dc.subject.mesh	Cell Adhesion	en_US
dc.subject.mesh	Cell Survival	en_US
dc.subject.mesh	Lab-On-A-Chip Devices	en_US
dc.subject.mesh	Single-Cell Analysis	en_US
dc.subject.mesh	Cellular Microenvironment	en_US
dc.title	Static droplet array for culturing single live adherent cells in an isolated chemical microenvironment	en_US
dc.type	Journal Article
utslib.citation.volume	15	en_US
utslib.citation.volume	18	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	09 Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access	*
pubs.issue	15	en_US
pubs.publication-status	Published	en_US
pubs.volume	18	en_US

Abstract:

© 2018 The Royal Society of Chemistry. We present here a new method to easily and reliably generate an array of hundreds of dispersed nanoliter-volume semi-droplets for single-cells culture and analysis. The liquid segmentation step occurs directly in indexed traps by a tweezer-like mechanism and is stabilized by spatial confinement. Unlike common droplet-based techniques, the semi-droplet wets its surrounding trap walls thus supporting the culturing of both adherent and non-adherent cells. To eliminate cross-droplet cell migration and chemical cross-talk each semi-droplet is separated from a nearby trap by an ∼80 pL air plug. The overall setup and injection procedure takes less than 10 minutes, is insensitive to fabrication defects and supports cell recovery for downstream analysis. The method offers a new approach to easily capture, image and culture single cells in a chemically isolated microenvironment as a preliminary step towards high-throughput single-cell assays.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/132023