A coarse-grained red blood cell membrane model to study stomatocyte-discocyteechinocyte morphologies

Geekiyanage, NM; Balanant, MA; Sauret, E; Saha, S; Flower, R; Lim, CT; Gu, YT

A coarse-grained red blood cell membrane model to study stomatocyte-discocyteechinocyte morphologies

Geekiyanage, NM Balanant, MA Sauret, E Saha, S

Flower, R Lim, CT Gu, YT

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Publication Type:: Journal Article
Citation:: PLoS ONE, 2019, 14 (4)
Issue Date:: 2019-04-01

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Field	Value	Language
dc.contributor.author	Geekiyanage, NM	en_US
dc.contributor.author	Balanant, MA	en_US
dc.contributor.author	Sauret, E	en_US
dc.contributor.author	Saha, S https://orcid.org/0000-0002-9962-8919	en_US
dc.contributor.author	Flower, R	en_US
dc.contributor.author	Lim, CT	en_US
dc.contributor.author	Gu, YT	en_US
dc.date.available	2019-04-02	en_US
dc.date.issued	2019-04-01	en_US
dc.identifier.citation	PLoS ONE, 2019, 14 (4)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/134527
dc.description.abstract	© 2019 Geekiyanage et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. An improved red blood cell (RBC) membrane model is developed based on the bilayer coupling model (BCM) to accurately predict the complete sequence of stomatocyte-discocyteechinocyte (SDE) transformation of a RBC. The coarse-grained (CG)-RBC membrane model is proposed to predict the minimum energy configuration of the RBC from the competition between lipid-bilayer bending resistance and cytoskeletal shear resistance under given reference constraints. In addition to the conventional membrane surface area, cell volume and bilayer-leaflet-area-difference constraints, a new constraint: Total-membrane-curvature is proposed in the model to better predict RBC shapes in agreement with experimental observations. A quantitative evaluation of several cellular measurements including length, thickness and shape factor, is performed for the first time, between CGRBC model predicted and three-dimensional (3D) confocal microscopy imaging generated RBC shapes at equivalent reference constraints. The validated CG-RBC membrane model is then employed to investigate the effect of reduced cell volume and elastic length scale on SDE transformation, to evaluate the RBC deformability during SDE transformation, and to identify the most probable RBC cytoskeletal reference state. The CG-RBC membrane model can predict the SDE shape behaviour under diverse shape-transforming scenarios, in-vitro RBC storage, microvascular circulation and flow through microfluidic devices.	en_US
dc.relation	http://purl.org/au-research/grants/arc/LP150100737
dc.relation.ispartof	PLoS ONE	en_US
dc.relation.isbasedon	10.1371/journal.pone.0215447	en_US
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	General Science & Technology	en_US
dc.subject.mesh	Erythrocytes	en_US
dc.subject.mesh	Erythrocyte Membrane	en_US
dc.subject.mesh	Erythrocytes, Abnormal	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Microscopy, Electron, Scanning	en_US
dc.subject.mesh	Cell Size	en_US
dc.subject.mesh	Erythrocyte Deformability	en_US
dc.subject.mesh	Algorithms	en_US
dc.subject.mesh	Models, Biological	en_US
dc.subject.mesh	Biomechanical Phenomena	en_US
dc.title	A coarse-grained red blood cell membrane model to study stomatocyte-discocyteechinocyte morphologies	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	14	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Mechanical and Mechatronic Engineering
utslib.copyright.status	open_access	*
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	14	en_US

Abstract:

© 2019 Geekiyanage et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. An improved red blood cell (RBC) membrane model is developed based on the bilayer coupling model (BCM) to accurately predict the complete sequence of stomatocyte-discocyteechinocyte (SDE) transformation of a RBC. The coarse-grained (CG)-RBC membrane model is proposed to predict the minimum energy configuration of the RBC from the competition between lipid-bilayer bending resistance and cytoskeletal shear resistance under given reference constraints. In addition to the conventional membrane surface area, cell volume and bilayer-leaflet-area-difference constraints, a new constraint: Total-membrane-curvature is proposed in the model to better predict RBC shapes in agreement with experimental observations. A quantitative evaluation of several cellular measurements including length, thickness and shape factor, is performed for the first time, between CGRBC model predicted and three-dimensional (3D) confocal microscopy imaging generated RBC shapes at equivalent reference constraints. The validated CG-RBC membrane model is then employed to investigate the effect of reduced cell volume and elastic length scale on SDE transformation, to evaluate the RBC deformability during SDE transformation, and to identify the most probable RBC cytoskeletal reference state. The CG-RBC membrane model can predict the SDE shape behaviour under diverse shape-transforming scenarios, in-vitro RBC storage, microvascular circulation and flow through microfluidic devices.

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http://hdl.handle.net/10453/134527