Detection of proteoglycan loss from articular cartilage using Brillouin microscopy, with applications to osteoarthritis

Wu, PJ; Masouleh, MI; Dini, D; Paterson, C; Török, P; Overby, DR; Kabakova, IV

Detection of proteoglycan loss from articular cartilage using Brillouin microscopy, with applications to osteoarthritis

Wu, PJ Masouleh, MI Dini, D Paterson, C Török, P Overby, DR Kabakova, IV

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Publication Type:: Journal Article
Citation:: Biomedical Optics Express, 2019, 10 (5), pp. 2457 - 2466
Issue Date:: 2019-05-01

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Field	Value	Language
dc.contributor.author	Wu, PJ	en_US
dc.contributor.author	Masouleh, MI	en_US
dc.contributor.author	Dini, D	en_US
dc.contributor.author	Paterson, C	en_US
dc.contributor.author	Török, P	en_US
dc.contributor.author	Overby, DR	en_US
dc.contributor.author	Kabakova, IV https://orcid.org/0000-0002-6831-9478	en_US
dc.date.available	2019-02-22	en_US
dc.date.issued	2019-05-01	en_US
dc.identifier.citation	Biomedical Optics Express, 2019, 10 (5), pp. 2457 - 2466	en_US
dc.identifier.uri	http://hdl.handle.net/10453/135080
dc.description.abstract	© 2019, OSA - The Optical Society. All rights reserved. The degeneration of articular cartilage (AC) occurs in osteoarthritis (OA), which is a leading cause of pain and disability in middle-aged and older people. The early diseaserelated changes in cartilage extra-cellular matrix (ECM) start with depletion of proteoglycan (PG), leading to an increase in tissue hydration and permeability. These early compositional changes are small (<10%) and hence difficult to register with conventional non-invasive imaging technologies (magnetic resonance and ultrasound imaging). Here we apply Brillouin microscopy for detecting changes in the mechanical properties and composition of porcine AC. OA-like degradation is mimicked by enzymatic tissue digestion, and we compare Brillouin microscopy measurements against histological staining of PG depletion over varying digestion times and enzyme concentrations. The non-destructive nature of Brillouin imaging technology opens new avenues for creating minimally invasive arthroscopic devices for OA diagnostics and therapeutic monitoring.	en_US
dc.relation	http://purl.org/au-research/grants/arc/DP190101973
dc.relation.ispartof	Biomedical Optics Express	en_US
dc.relation.isbasedon	10.1364/BOE.10.002457	en_US
dc.title	Detection of proteoglycan loss from articular cartilage using Brillouin microscopy, with applications to osteoarthritis	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	10	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	0205 Optical Physics	en_US
utslib.for	0912 Materials Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	10	en_US

Abstract:

© 2019, OSA - The Optical Society. All rights reserved. The degeneration of articular cartilage (AC) occurs in osteoarthritis (OA), which is a leading cause of pain and disability in middle-aged and older people. The early diseaserelated changes in cartilage extra-cellular matrix (ECM) start with depletion of proteoglycan (PG), leading to an increase in tissue hydration and permeability. These early compositional changes are small (<10%) and hence difficult to register with conventional non-invasive imaging technologies (magnetic resonance and ultrasound imaging). Here we apply Brillouin microscopy for detecting changes in the mechanical properties and composition of porcine AC. OA-like degradation is mimicked by enzymatic tissue digestion, and we compare Brillouin microscopy measurements against histological staining of PG depletion over varying digestion times and enzyme concentrations. The non-destructive nature of Brillouin imaging technology opens new avenues for creating minimally invasive arthroscopic devices for OA diagnostics and therapeutic monitoring.

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http://hdl.handle.net/10453/135080