Towards engineering heart tissues from bioprinted cardiac spheroids.
Polonchuk, L
Surija, L
Lee, MH
Sharma, P
Liu Chung Ming, C
Richter, F
Ben-Sefer, E
Alsadat Rad, M
Mahmodi Sheikh Sarmast, H
Al Shamery, W
Tran, HA
Vettori, L
Haeusermann, F
Filipe, EC
Rnjak-Kovacina, J
Cox, T
Tipper, J
Kabakova, I
Gentile, C
- Publisher:
- IOP PUBLISHING LTD
- Publication Type:
- Journal Article
- Citation:
- Biofabrication, 2021, 13, (4)
- Issue Date:
- 2021-07-15
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Polonchuk, L | |
dc.contributor.author | Surija, L | |
dc.contributor.author | Lee, MH | |
dc.contributor.author | Sharma, P | |
dc.contributor.author | Liu Chung Ming, C | |
dc.contributor.author | Richter, F | |
dc.contributor.author | Ben-Sefer, E | |
dc.contributor.author | Alsadat Rad, M | |
dc.contributor.author |
Mahmodi Sheikh Sarmast, H https://orcid.org/0000-0003-4820-5614 |
|
dc.contributor.author | Al Shamery, W | |
dc.contributor.author | Tran, HA | |
dc.contributor.author | Vettori, L | |
dc.contributor.author | Haeusermann, F | |
dc.contributor.author | Filipe, EC | |
dc.contributor.author | Rnjak-Kovacina, J | |
dc.contributor.author | Cox, T | |
dc.contributor.author | Tipper, J | |
dc.contributor.author |
Kabakova, I https://orcid.org/0000-0002-6831-9478 |
|
dc.contributor.author |
Gentile, C https://orcid.org/0000-0002-3689-4275 |
|
dc.date.accessioned | 2021-11-29T11:02:47Z | |
dc.date.available | 2021-07-15 | |
dc.date.available | 2021-11-29T11:02:47Z | |
dc.date.issued | 2021-07-15 | |
dc.identifier.citation | Biofabrication, 2021, 13, (4) | |
dc.identifier.issn | 1758-5082 | |
dc.identifier.issn | 1758-5090 | |
dc.identifier.uri | http://hdl.handle.net/10453/151913 | |
dc.description.abstract | Current<i>in vivo</i>and<i>in vitro</i>models fail to accurately recapitulate the human heart microenvironment for biomedical applications. This study explores the use of cardiac spheroids (CSs) to biofabricate advanced<i>in vitro</i>models of the human heart. CSs were created from human cardiac myocytes, fibroblasts and endothelial cells (ECs), mixed within optimal alginate/gelatin hydrogels and then bioprinted on a microelectrode plate for drug testing. Bioprinted CSs maintained their structure and viability for at least 30 d after printing. Vascular endothelial growth factor (VEGF) promoted EC branching from CSs within hydrogels. Alginate/gelatin-based hydrogels enabled spheroids fusion, which was further facilitated by addition of VEGF. Bioprinted CSs contracted spontaneously and under stimulation, allowing to record contractile and electrical signals on the microelectrode plates for industrial applications. Taken together, our findings indicate that bioprinted CSs can be used to biofabricate human heart tissues for long term<i>in vitro</i>testing. This has the potential to be used to study biochemical, physiological and pharmacological features of human heart tissue. | |
dc.format | Electronic | |
dc.language | eng | |
dc.publisher | IOP PUBLISHING LTD | |
dc.relation | http://purl.org/au-research/grants/arc/DP190101973 | |
dc.relation.ispartof | Biofabrication | |
dc.relation.isbasedon | 10.1088/1758-5090/ac14ca | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | 0903 Biomedical Engineering, 1004 Medical Biotechnology, 1099 Other Technology | |
dc.title | Towards engineering heart tissues from bioprinted cardiac spheroids. | |
dc.type | Journal Article | |
utslib.citation.volume | 13 | |
utslib.location.activity | England | |
utslib.for | 0903 Biomedical Engineering | |
utslib.for | 1004 Medical Biotechnology | |
utslib.for | 1099 Other Technology | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Strength - CHT - Health Technologies | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
pubs.organisational-group | /University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices | |
pubs.organisational-group | /University of Technology Sydney/Centre for Health Technologies (CHT) | |
utslib.copyright.status | open_access | * |
dc.date.updated | 2021-11-29T11:02:41Z | |
pubs.issue | 4 | |
pubs.publication-status | Published | |
pubs.volume | 13 | |
utslib.citation.issue | 4 |
Abstract:
Currentin vivoandin vitromodels fail to accurately recapitulate the human heart microenvironment for biomedical applications. This study explores the use of cardiac spheroids (CSs) to biofabricate advancedin vitromodels of the human heart. CSs were created from human cardiac myocytes, fibroblasts and endothelial cells (ECs), mixed within optimal alginate/gelatin hydrogels and then bioprinted on a microelectrode plate for drug testing. Bioprinted CSs maintained their structure and viability for at least 30 d after printing. Vascular endothelial growth factor (VEGF) promoted EC branching from CSs within hydrogels. Alginate/gelatin-based hydrogels enabled spheroids fusion, which was further facilitated by addition of VEGF. Bioprinted CSs contracted spontaneously and under stimulation, allowing to record contractile and electrical signals on the microelectrode plates for industrial applications. Taken together, our findings indicate that bioprinted CSs can be used to biofabricate human heart tissues for long termin vitrotesting. This has the potential to be used to study biochemical, physiological and pharmacological features of human heart tissue.
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