| Field |
Value |
Language |
|
dc.contributor.author |
Rezaei, M |
|
|
dc.contributor.author |
Razavi Bazaz, S
https://orcid.org/0000-0002-6419-3361
|
|
|
dc.contributor.author |
Morshedi Rad, D |
|
|
dc.contributor.author |
Shimoni, O
https://orcid.org/0000-0001-8822-1024
|
|
|
dc.contributor.author |
Jin, D
https://orcid.org/0000-0003-1046-2666
|
|
|
dc.contributor.author |
Rawlinson, W |
|
|
dc.contributor.author |
Ebrahimi Warkiani, M
https://orcid.org/0000-0002-4184-1944
|
|
|
dc.date.accessioned |
2022-01-07T00:33:53Z |
|
|
dc.date.available |
2021-09-28 |
|
|
dc.date.available |
2022-01-07T00:33:53Z |
|
|
dc.date.issued |
2021-10-02 |
|
|
dc.identifier.citation |
Biosensors (Basel), 2021, 11, (10), pp. 369 |
|
|
dc.identifier.issn |
2079-6374 |
|
|
dc.identifier.issn |
2079-6374 |
|
|
dc.identifier.uri |
http://hdl.handle.net/10453/152795
|
|
|
dc.description.abstract |
The COVID-19 pandemic has changed people's lives and has brought society to a sudden standstill, with lockdowns and social distancing as the preferred preventative measures. To lift these measurements and reduce society's burden, developing an easy-to-use, rapid, and portable system to detect SARS-CoV-2 is mandatory. To this end, we developed a portable and semi-automated device for SARS-CoV-2 detection based on reverse transcription loop-mediated isothermal amplification followed by a CRISPR/Cas12a reaction. The device contains a heater element mounted on a printed circuit board, a cooler fan, a proportional integral derivative controller to control the temperature, and designated areas for 0.2 mL Eppendorf® PCR tubes. Our system has a limit of detection of 35 copies of the virus per microliter, which is significant and has the capability of being used in crisis centers, mobile laboratories, remote locations, or airports to diagnose individuals infected with SARS-CoV-2. We believe the current methodology that we have implemented in this article is beneficial for the early screening of infectious diseases, in which fast screening with high accuracy is necessary. |
|
|
dc.format |
Electronic |
|
|
dc.language |
eng |
|
|
dc.publisher |
MDPI AG |
|
|
dc.relation |
http://purl.org/au-research/grants/arc/DP170103704
|
|
|
dc.relation |
http://purl.org/au-research/grants/arc/DP180103003
|
|
|
dc.relation |
http://purl.org/au-research/grants/nhmrc/1143377
|
|
|
dc.relation |
http://purl.org/au-research/grants/arc/IH150100028
|
|
|
dc.relation.ispartof |
Biosensors (Basel) |
|
|
dc.relation.isbasedon |
10.3390/bios11100369 |
|
|
dc.rights |
info:eu-repo/semantics/openAccess |
|
|
dc.rights |
Copyright: © 2021 by the authors.
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
conditions of the Creative Commons
Attribution (CC BY) license (https://
creativecommons.org/licenses/by/
4.0/). |
|
|
dc.subject |
0301 Analytical Chemistry, 0601 Biochemistry and Cell Biology |
|
|
dc.subject.mesh |
COVID-19 |
|
|
dc.subject.mesh |
COVID-19 Testing |
|
|
dc.subject.mesh |
CRISPR-Cas Systems |
|
|
dc.subject.mesh |
Humans |
|
|
dc.subject.mesh |
Limit of Detection |
|
|
dc.subject.mesh |
Molecular Diagnostic Techniques |
|
|
dc.subject.mesh |
Nucleic Acid Amplification Techniques |
|
|
dc.subject.mesh |
Point-of-Care Systems |
|
|
dc.subject.mesh |
RNA, Viral |
|
|
dc.subject.mesh |
SARS-CoV-2 |
|
|
dc.subject.mesh |
Humans |
|
|
dc.subject.mesh |
RNA, Viral |
|
|
dc.subject.mesh |
Molecular Diagnostic Techniques |
|
|
dc.subject.mesh |
Nucleic Acid Amplification Techniques |
|
|
dc.subject.mesh |
Point-of-Care Systems |
|
|
dc.subject.mesh |
Limit of Detection |
|
|
dc.subject.mesh |
CRISPR-Cas Systems |
|
|
dc.subject.mesh |
COVID-19 |
|
|
dc.subject.mesh |
SARS-CoV-2 |
|
|
dc.subject.mesh |
COVID-19 Testing |
|
|
dc.title |
A Portable RT-LAMP/CRISPR Machine for Rapid COVID-19 Screening. |
|
|
dc.type |
Journal Article |
|
|
utslib.citation.volume |
11 |
|
|
utslib.location.activity |
Switzerland |
|
|
utslib.for |
0301 Analytical Chemistry |
|
|
utslib.for |
0601 Biochemistry and Cell Biology |
|
|
pubs.organisational-group |
/University of Technology Sydney |
|
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Engineering and Information Technology |
|
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Science |
|
|
pubs.organisational-group |
/University of Technology Sydney/Strength - CHT - Health Technologies |
|
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences |
|
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering |
|
|
pubs.organisational-group |
/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices |
|
|
pubs.organisational-group |
/University of Technology Sydney/Centre for Health Technologies (CHT) |
|
|
utslib.copyright.status |
open_access |
* |
|
dc.date.updated |
2022-01-07T00:33:51Z |
|
|
pubs.issue |
10 |
|
|
pubs.publication-status |
Published online |
|
|
pubs.volume |
11 |
|
|
utslib.citation.issue |
10 |
|
