Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue.

Erami, Z; Herrmann, D; Warren, SC; Nobis, M; McGhee, EJ; Lucas, MC; Leung, W; Reischmann, N; Mrowinska, A; Schwarz, JP; Kadir, S; Conway, JRW; Vennin, C; Karim, SA; Campbell, AD; Gallego-Ortega, D; Magenau, A; Murphy, KJ; Ridgway, RA; Law, AM; Walters, SN; Grey, ST; Croucher, DR; Zhang, L; Herzog, H; Hardeman, EC; Gunning, PW; Ormandy, CJ; Evans, TRJ; Strathdee, D; Sansom, OJ; Morton, JP; Anderson, KI; Timpson, P

Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue.

Erami, Z Herrmann, D Warren, SC Nobis, M McGhee, EJ Lucas, MC Leung, W Reischmann, N Mrowinska, A Schwarz, JP Kadir, S Conway, JRW Vennin, C Karim, SA Campbell, AD Gallego-Ortega, D Magenau, A Murphy, KJ Ridgway, RA Law, AM Walters, SN Grey, ST Croucher, DR Zhang, L Herzog, H Hardeman, EC Gunning, PW Ormandy, CJ Evans, TRJ Strathdee, D Sansom, OJ Morton, JP Anderson, KI Timpson, P

Permalink

Publisher:: CELL PRESS
Publication Type:: Journal Article
Citation:: Cell Rep, 2016, 14, (1), pp. 152-167
Issue Date:: 2016-01-05

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download full textAdobe PDF (13.77 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Erami, Z
dc.contributor.author	Herrmann, D
dc.contributor.author	Warren, SC
dc.contributor.author	Nobis, M
dc.contributor.author	McGhee, EJ
dc.contributor.author	Lucas, MC
dc.contributor.author	Leung, W
dc.contributor.author	Reischmann, N
dc.contributor.author	Mrowinska, A
dc.contributor.author	Schwarz, JP
dc.contributor.author	Kadir, S
dc.contributor.author	Conway, JRW
dc.contributor.author	Vennin, C
dc.contributor.author	Karim, SA
dc.contributor.author	Campbell, AD
dc.contributor.author	Gallego-Ortega, D
dc.contributor.author	Magenau, A
dc.contributor.author	Murphy, KJ
dc.contributor.author	Ridgway, RA
dc.contributor.author	Law, AM
dc.contributor.author	Walters, SN
dc.contributor.author	Grey, ST
dc.contributor.author	Croucher, DR
dc.contributor.author	Zhang, L
dc.contributor.author	Herzog, H
dc.contributor.author	Hardeman, EC
dc.contributor.author	Gunning, PW
dc.contributor.author	Ormandy, CJ
dc.contributor.author	Evans, TRJ
dc.contributor.author	Strathdee, D
dc.contributor.author	Sansom, OJ
dc.contributor.author	Morton, JP
dc.contributor.author	Anderson, KI
dc.contributor.author	Timpson, P
dc.date.accessioned	2022-04-13T12:32:09Z
dc.date.available	2015-11-23
dc.date.available	2022-04-13T12:32:09Z
dc.date.issued	2016-01-05
dc.identifier.citation	Cell Rep, 2016, 14, (1), pp. 152-167
dc.identifier.issn	2211-1247
dc.identifier.issn	2211-1247
dc.identifier.uri	http://hdl.handle.net/10453/156224
dc.description.abstract	E-cadherin-mediated cell-cell junctions play a prominent role in maintaining the epithelial architecture. The disruption or deregulation of these adhesions in cancer can lead to the collapse of tumor epithelia that precedes invasion and subsequent metastasis. Here we generated an E-cadherin-GFP mouse that enables intravital photobleaching and quantification of E-cadherin mobility in live tissue without affecting normal biology. We demonstrate the broad applications of this mouse by examining E-cadherin regulation in multiple tissues, including mammary, brain, liver, and kidney tissue, while specifically monitoring E-cadherin mobility during disease progression in the pancreas. We assess E-cadherin stability in native pancreatic tissue upon genetic manipulation involving Kras and p53 or in response to anti-invasive drug treatment and gain insights into the dynamic remodeling of E-cadherin during in situ cancer progression. FRAP in the E-cadherin-GFP mouse, therefore, promises to be a valuable tool to fundamentally expand our understanding of E-cadherin-mediated events in native microenvironments.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	CELL PRESS
dc.relation.ispartof	Cell Rep
dc.relation.isbasedon	10.1016/j.celrep.2015.12.020
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0601 Biochemistry and Cell Biology, 1116 Medical Physiology
dc.subject.mesh	Animals
dc.subject.mesh	Cadherins
dc.subject.mesh	Green Fluorescent Proteins
dc.subject.mesh	Mice
dc.subject.mesh	Mice, Transgenic
dc.subject.mesh	Neoplasms, Experimental
dc.subject.mesh	Optical Imaging
dc.subject.mesh	Organ Specificity
dc.subject.mesh	Proto-Oncogene Proteins p21(ras)
dc.subject.mesh	Tumor Microenvironment
dc.subject.mesh	Tumor Suppressor Protein p53
dc.subject.mesh	Animals
dc.subject.mesh	Mice, Transgenic
dc.subject.mesh	Mice
dc.subject.mesh	Neoplasms, Experimental
dc.subject.mesh	Cadherins
dc.subject.mesh	Green Fluorescent Proteins
dc.subject.mesh	Organ Specificity
dc.subject.mesh	Tumor Suppressor Protein p53
dc.subject.mesh	Proto-Oncogene Proteins p21(ras)
dc.subject.mesh	Tumor Microenvironment
dc.subject.mesh	Optical Imaging
dc.title	Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue.
dc.type	Journal Article
utslib.citation.volume	14
utslib.location.activity	United States
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	1116 Medical Physiology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2022-04-13T12:32:01Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	14
utslib.citation.issue	1

Abstract:

E-cadherin-mediated cell-cell junctions play a prominent role in maintaining the epithelial architecture. The disruption or deregulation of these adhesions in cancer can lead to the collapse of tumor epithelia that precedes invasion and subsequent metastasis. Here we generated an E-cadherin-GFP mouse that enables intravital photobleaching and quantification of E-cadherin mobility in live tissue without affecting normal biology. We demonstrate the broad applications of this mouse by examining E-cadherin regulation in multiple tissues, including mammary, brain, liver, and kidney tissue, while specifically monitoring E-cadherin mobility during disease progression in the pancreas. We assess E-cadherin stability in native pancreatic tissue upon genetic manipulation involving Kras and p53 or in response to anti-invasive drug treatment and gain insights into the dynamic remodeling of E-cadherin during in situ cancer progression. FRAP in the E-cadherin-GFP mouse, therefore, promises to be a valuable tool to fundamentally expand our understanding of E-cadherin-mediated events in native microenvironments.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/156224