An Acoustic Platform for Single-Cell, High-Throughput Measurements of the Viscoelastic Properties of Cells.

Romanov, V; Silvani, G; Zhu, H; Cox, CD; Martinac, B

An Acoustic Platform for Single-Cell, High-Throughput Measurements of the Viscoelastic Properties of Cells.

Romanov, V Silvani, G

Zhu, H Cox, CD Martinac, B

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Publisher:: WILEY-V C H VERLAG GMBH
Publication Type:: Journal Article
Citation:: Small, 2021, 17, (3), pp. e2005759
Issue Date:: 2021-01

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Field	Value	Language
dc.contributor.author	Romanov, V
dc.contributor.author	Silvani, G https://orcid.org/0000-0001-6661-2817
dc.contributor.author	Zhu, H
dc.contributor.author	Cox, CD
dc.contributor.author	Martinac, B
dc.date.accessioned	2022-05-17T00:43:33Z
dc.date.available	2022-05-17T00:43:33Z
dc.date.issued	2021-01
dc.identifier.citation	Small, 2021, 17, (3), pp. e2005759
dc.identifier.issn	1613-6810
dc.identifier.issn	1613-6829
dc.identifier.uri	http://hdl.handle.net/10453/157439
dc.description.abstract	Cellular processes including adhesion, migration, and differentiation are governed by the distinct mechanical properties of each cell. Importantly, the mechanical properties of individual cells can vary depending on local physical and biochemical cues in a time-dependent manner resulting in significant inter-cell heterogeneity. While several different methods have been developed to interrogate the mechanical properties of single cells, throughput to capture this heterogeneity remains an issue. Here, single-cell, high-throughput characterization of adherent cells is demonstrated using acoustic force spectroscopy (AFS). AFS works by simultaneously, acoustically driving tens to hundreds of silica beads attached to cells away from the cell surface, allowing the user to measure the stiffness of adherent cells under multiple experimental conditions. It is shown that cells undergo marked changes in viscoelasticity as a function of temperature, by altering the temperature within the AFS microfluidic circuit between 21 and 37 °C. In addition, quantitative differences in cells exposed to different pharmacological treatments specifically targeting the membrane-cytoskeleton interface are shown. Further, the high-throughput format of the AFS is utilized to rapidly probe, in excess of 1000 cells, three different cell lines expressing different levels of a mechanosensitive protein, Piezo1, demonstrating the ability to differentiate between cells based on protein expression levels.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY-V C H VERLAG GMBH
dc.relation.ispartof	Small
dc.relation.isbasedon	10.1002/smll.202005759
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Nanoscience & Nanotechnology
dc.subject.mesh	Acoustics
dc.subject.mesh	Cytoskeleton
dc.subject.mesh	Elasticity
dc.subject.mesh	Mechanical Phenomena
dc.subject.mesh	Microfluidics
dc.subject.mesh	Viscosity
dc.subject.mesh	Cytoskeleton
dc.subject.mesh	Microfluidics
dc.subject.mesh	Viscosity
dc.subject.mesh	Acoustics
dc.subject.mesh	Elasticity
dc.subject.mesh	Mechanical Phenomena
dc.title	An Acoustic Platform for Single-Cell, High-Throughput Measurements of the Viscoelastic Properties of Cells.
dc.type	Journal Article
utslib.citation.volume	17
utslib.location.activity	Germany
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2022-05-17T00:43:29Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	17
utslib.citation.issue	3

Abstract:

Cellular processes including adhesion, migration, and differentiation are governed by the distinct mechanical properties of each cell. Importantly, the mechanical properties of individual cells can vary depending on local physical and biochemical cues in a time-dependent manner resulting in significant inter-cell heterogeneity. While several different methods have been developed to interrogate the mechanical properties of single cells, throughput to capture this heterogeneity remains an issue. Here, single-cell, high-throughput characterization of adherent cells is demonstrated using acoustic force spectroscopy (AFS). AFS works by simultaneously, acoustically driving tens to hundreds of silica beads attached to cells away from the cell surface, allowing the user to measure the stiffness of adherent cells under multiple experimental conditions. It is shown that cells undergo marked changes in viscoelasticity as a function of temperature, by altering the temperature within the AFS microfluidic circuit between 21 and 37 °C. In addition, quantitative differences in cells exposed to different pharmacological treatments specifically targeting the membrane-cytoskeleton interface are shown. Further, the high-throughput format of the AFS is utilized to rapidly probe, in excess of 1000 cells, three different cell lines expressing different levels of a mechanosensitive protein, Piezo1, demonstrating the ability to differentiate between cells based on protein expression levels.

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http://hdl.handle.net/10453/157439