Ultra-fast, label-free isolation of circulating tumor cells from blood using spiral microfluidics.
- Publisher:
- NATURE RESEARCH
- Publication Type:
- Journal Article
- Citation:
- Nat Protoc, 2016, 11, (1), pp. 134-148
- Issue Date:
- 2016-01
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Ultra-fast,_label-free_isolati.pdf | Published version | 2.61 MB | Adobe PDF |
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Warkiani, ME | |
dc.contributor.author | Khoo, BL | |
dc.contributor.author | Wu, L | |
dc.contributor.author | Tay, AKP | |
dc.contributor.author | Bhagat, AAS | |
dc.contributor.author | Han, J | |
dc.contributor.author | Lim, CT | |
dc.date.accessioned | 2022-07-13T00:13:39Z | |
dc.date.available | 2022-07-13T00:13:39Z | |
dc.date.issued | 2016-01 | |
dc.identifier.citation | Nat Protoc, 2016, 11, (1), pp. 134-148 | |
dc.identifier.issn | 1754-2189 | |
dc.identifier.issn | 1750-2799 | |
dc.identifier.uri | http://hdl.handle.net/10453/158819 | |
dc.description.abstract | Circulating tumor cells (CTCs) are rare cancer cells that are shed from primary or metastatic tumors into the peripheral blood circulation. Phenotypic and genetic characterization of these rare cells can provide important information to guide cancer staging and treatment, and thus further research into their characteristics and properties is an area of considerable interest. In this protocol, we describe detailed procedures for the production and use of a label-free spiral microfluidic device to allow size-based isolation of viable CTCs using hydrodynamic forces that are present in curvilinear microchannels. This spiral system enables us to achieve ≥ 85% recovery of spiked cells across multiple cancer cell lines and 99.99% depletion of white blood cells in whole blood. The described spiral microfluidic devices can be produced at an extremely low cost using standard microfabrication and soft lithography techniques (2-3 d), and they can be operated using two syringe pumps for lysed blood samples (7.5 ml in 12.5 min for a three-layered multiplexed chip). The fast processing time and the ability to collect CTCs from a large patient blood volume allows this technique to be used experimentally in a broad range of potential genomic and transcriptomic applications. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | NATURE RESEARCH | |
dc.relation.ispartof | Nat Protoc | |
dc.relation.isbasedon | 10.1038/nprot.2016.003 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | 03 Chemical Sciences, 06 Biological Sciences, 11 Medical and Health Sciences | |
dc.subject.classification | Bioinformatics | |
dc.subject.mesh | Cell Death | |
dc.subject.mesh | Cell Line, Tumor | |
dc.subject.mesh | Cell Separation | |
dc.subject.mesh | Equipment Design | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Lab-On-A-Chip Devices | |
dc.subject.mesh | Microspheres | |
dc.subject.mesh | Neoplastic Cells, Circulating | |
dc.subject.mesh | Time Factors | |
dc.subject.mesh | Cell Line, Tumor | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Cell Separation | |
dc.subject.mesh | Equipment Design | |
dc.subject.mesh | Microspheres | |
dc.subject.mesh | Cell Death | |
dc.subject.mesh | Time Factors | |
dc.subject.mesh | Neoplastic Cells, Circulating | |
dc.subject.mesh | Lab-On-A-Chip Devices | |
dc.title | Ultra-fast, label-free isolation of circulating tumor cells from blood using spiral microfluidics. | |
dc.type | Journal Article | |
utslib.citation.volume | 11 | |
utslib.location.activity | England | |
utslib.for | 03 Chemical Sciences | |
utslib.for | 06 Biological Sciences | |
utslib.for | 11 Medical and Health Sciences | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Strength - CHT - Health Technologies | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
pubs.organisational-group | /University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices | |
pubs.organisational-group | /University of Technology Sydney/Centre for Health Technologies (CHT) | |
utslib.copyright.status | closed_access | * |
dc.date.updated | 2022-07-13T00:13:38Z | |
pubs.issue | 1 | |
pubs.publication-status | Published | |
pubs.volume | 11 | |
utslib.citation.issue | 1 |
Abstract:
Circulating tumor cells (CTCs) are rare cancer cells that are shed from primary or metastatic tumors into the peripheral blood circulation. Phenotypic and genetic characterization of these rare cells can provide important information to guide cancer staging and treatment, and thus further research into their characteristics and properties is an area of considerable interest. In this protocol, we describe detailed procedures for the production and use of a label-free spiral microfluidic device to allow size-based isolation of viable CTCs using hydrodynamic forces that are present in curvilinear microchannels. This spiral system enables us to achieve ≥ 85% recovery of spiked cells across multiple cancer cell lines and 99.99% depletion of white blood cells in whole blood. The described spiral microfluidic devices can be produced at an extremely low cost using standard microfabrication and soft lithography techniques (2-3 d), and they can be operated using two syringe pumps for lysed blood samples (7.5 ml in 12.5 min for a three-layered multiplexed chip). The fast processing time and the ability to collect CTCs from a large patient blood volume allows this technique to be used experimentally in a broad range of potential genomic and transcriptomic applications.
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