Micropatterning of porous silicon Bragg reflectors with poly(ethylene glycol) to fabricate cell microarrays: Towards single cell sensing.
- Publisher:
- ELSEVIER ADVANCED TECHNOLOGY
- Publication Type:
- Journal Article
- Citation:
- Biosens Bioelectron, 2019, 127, pp. 229-235
- Issue Date:
- 2019-02-15
Closed Access
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1-s2.0-S0956566318309461-main.pdf | Published version | 4.17 MB | Adobe PDF |
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Piya, R | |
dc.contributor.author |
Zhu, Y |
|
dc.contributor.author | Soeriyadi, AH | |
dc.contributor.author | Silva, SM | |
dc.contributor.author | Reece, PJ | |
dc.contributor.author | Gooding, JJ | |
dc.date.accessioned | 2022-07-22T05:37:14Z | |
dc.date.available | 2018-12-02 | |
dc.date.available | 2022-07-22T05:37:14Z | |
dc.date.issued | 2019-02-15 | |
dc.identifier.citation | Biosens Bioelectron, 2019, 127, pp. 229-235 | |
dc.identifier.issn | 0956-5663 | |
dc.identifier.issn | 1873-4235 | |
dc.identifier.uri | http://hdl.handle.net/10453/159118 | |
dc.description.abstract | The work presented here describes the development of an optical label-free biosensor based on a porous silicon (PSi) Bragg reflector to study heterogeneity in single cells. Photolithographic patterning of a poly(ethylene glycol) (PEG) hydrogel with a photoinitiator was employed on RGD peptide-modified PSi to create micropatterns with cell adhesive and cell repellent areas. Macrophage J774 cells were incubated to form cell microarrays and single cell arrays. Moreover, cells on the microarrays were lysed osmotically with Milli-Q™ water and the infiltration of cell lysate into the porous matrix was monitored by measuring the red shift in the reflectivity. On average, the magnitude of red shift increased with the increase in the number of cells on the micropatterns. The red shift from the spots with single cells varied from spot to spot emphasizing the heterogeneous nature of the individual cells. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | |
dc.relation.ispartof | Biosens Bioelectron | |
dc.relation.isbasedon | 10.1016/j.bios.2018.12.001 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | 0301 Analytical Chemistry, 0903 Biomedical Engineering, 1007 Nanotechnology | |
dc.subject.classification | Bioinformatics | |
dc.subject.mesh | Biosensing Techniques | |
dc.subject.mesh | Hydrogels | |
dc.subject.mesh | Polyethylene Glycols | |
dc.subject.mesh | Porosity | |
dc.subject.mesh | Silicon | |
dc.subject.mesh | Single-Cell Analysis | |
dc.subject.mesh | Surface Properties | |
dc.subject.mesh | Tissue Array Analysis | |
dc.subject.mesh | Silicon | |
dc.subject.mesh | Polyethylene Glycols | |
dc.subject.mesh | Hydrogels | |
dc.subject.mesh | Tissue Array Analysis | |
dc.subject.mesh | Biosensing Techniques | |
dc.subject.mesh | Surface Properties | |
dc.subject.mesh | Porosity | |
dc.subject.mesh | Single-Cell Analysis | |
dc.title | Micropatterning of porous silicon Bragg reflectors with poly(ethylene glycol) to fabricate cell microarrays: Towards single cell sensing. | |
dc.type | Journal Article | |
utslib.citation.volume | 127 | |
utslib.location.activity | England | |
utslib.for | 0301 Analytical Chemistry | |
utslib.for | 0903 Biomedical Engineering | |
utslib.for | 1007 Nanotechnology | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
pubs.organisational-group | /University of Technology Sydney/Centre for Health Technologies (CHT) | |
utslib.copyright.status | closed_access | * |
dc.date.updated | 2022-07-22T05:37:07Z | |
pubs.publication-status | Published | |
pubs.volume | 127 |
Abstract:
The work presented here describes the development of an optical label-free biosensor based on a porous silicon (PSi) Bragg reflector to study heterogeneity in single cells. Photolithographic patterning of a poly(ethylene glycol) (PEG) hydrogel with a photoinitiator was employed on RGD peptide-modified PSi to create micropatterns with cell adhesive and cell repellent areas. Macrophage J774 cells were incubated to form cell microarrays and single cell arrays. Moreover, cells on the microarrays were lysed osmotically with Milli-Q™ water and the infiltration of cell lysate into the porous matrix was monitored by measuring the red shift in the reflectivity. On average, the magnitude of red shift increased with the increase in the number of cells on the micropatterns. The red shift from the spots with single cells varied from spot to spot emphasizing the heterogeneous nature of the individual cells.
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