Filling the Gaps between the In Vivo and In Vitro Microenvironment: Engineering of Spheroids for Stem Cell Technology.

Gentile, C

Filling the Gaps between the In Vivo and In Vitro Microenvironment: Engineering of Spheroids for Stem Cell Technology.

Gentile, C

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Publisher:: BENTHAM SCIENCE PUBL LTD
Publication Type:: Journal Article
Citation:: Curr Stem Cell Res Ther, 2016, 11, (8), pp. 652-665
Issue Date:: 2016

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Field	Value	Language
dc.contributor.author	Gentile, C https://orcid.org/0000-0002-3689-4275
dc.date.accessioned	2022-08-09T20:38:24Z
dc.date.available	2015-09-01
dc.date.available	2022-08-09T20:38:24Z
dc.date.issued	2016
dc.identifier.citation	Curr Stem Cell Res Ther, 2016, 11, (8), pp. 652-665
dc.identifier.issn	1574-888X
dc.identifier.issn	2212-3946
dc.identifier.uri	http://hdl.handle.net/10453/159831
dc.description.abstract	Engineering of in vitro three-dimensional cultures of stem cells and their progenies has offered promising alternatives to recapitulate the in vivo microenvironment, or stem cell niche, and has provided more specific cues for proper stem cell differentiation, maintenance and culture. In particular, tissue spheroids are cellular aggregates with defined cellular and extracellular features and have provided optimal conditions for stem cell technology, both in culture and for potential engraftment. Recent studies have focused on spheroid formation and the developmental roles played by cellular and extracellular signals necessary for cellular aggegation into spheroids. This review will provide insights into the factors that regulate in vitro spheroid formation by comparing them with their developmental counterparts in vivo. At the same time, we will identify cellular and extracellular signals that could be used to bioengineer spheroids with improved features according to their application. Finally, this review will provide an overview of the applications to date of spheroid cultures of stem cells and their progenies, providing insights for future studies.
dc.format	Print
dc.language	eng
dc.publisher	BENTHAM SCIENCE PUBL LTD
dc.relation.ispartof	Curr Stem Cell Res Ther
dc.relation.isbasedon	10.2174/1574888x10666151001114848
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0601 Biochemistry and Cell Biology, 1101 Medical Biochemistry and Metabolomics
dc.subject.classification	Immunology
dc.subject.mesh	Animals
dc.subject.mesh	Cellular Microenvironment
dc.subject.mesh	Humans
dc.subject.mesh	Regenerative Medicine
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Stem Cell Research
dc.subject.mesh	Stem Cells
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Stem Cells
dc.subject.mesh	Animals
dc.subject.mesh	Humans
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Regenerative Medicine
dc.subject.mesh	Stem Cell Research
dc.subject.mesh	Cellular Microenvironment
dc.title	Filling the Gaps between the In Vivo and In Vitro Microenvironment: Engineering of Spheroids for Stem Cell Technology.
dc.type	Journal Article
utslib.citation.volume	11
utslib.location.activity	United Arab Emirates
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	1101 Medical Biochemistry and Metabolomics
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2022-08-09T20:38:23Z
pubs.issue	8
pubs.publication-status	Published
pubs.volume	11
utslib.citation.issue	8

Abstract:

Engineering of in vitro three-dimensional cultures of stem cells and their progenies has offered promising alternatives to recapitulate the in vivo microenvironment, or stem cell niche, and has provided more specific cues for proper stem cell differentiation, maintenance and culture. In particular, tissue spheroids are cellular aggregates with defined cellular and extracellular features and have provided optimal conditions for stem cell technology, both in culture and for potential engraftment. Recent studies have focused on spheroid formation and the developmental roles played by cellular and extracellular signals necessary for cellular aggegation into spheroids. This review will provide insights into the factors that regulate in vitro spheroid formation by comparing them with their developmental counterparts in vivo. At the same time, we will identify cellular and extracellular signals that could be used to bioengineer spheroids with improved features according to their application. Finally, this review will provide an overview of the applications to date of spheroid cultures of stem cells and their progenies, providing insights for future studies.

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http://hdl.handle.net/10453/159831