Exploiting Dynamic Nonlinearity in Upconversion Nanoparticles for Super-Resolution Imaging.
Chen, C
Ding, L
Liu, B
Du, Z
Liu, Y
Di, X
Shan, X
Lin, C
Zhang, M
Xu, X
Zhong, X
Wang, J
Chang, L
Halkon, B
Chen, X
Cheng, F
Wang, F
- Publisher:
- AMER CHEMICAL SOC
- Publication Type:
- Journal Article
- Citation:
- Nano Lett, 2022, 22, (17), pp. 7136-7143
- Issue Date:
- 2022-09-14
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2022-Exploiting Dynamic Nonlinearity in Upconversion Nanoparticles for Super-Resolution Imaging.pdf | 4.77 MB | Adobe PDF |
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author |
Chen, C |
|
dc.contributor.author | Ding, L | |
dc.contributor.author | Liu, B | |
dc.contributor.author | Du, Z | |
dc.contributor.author | Liu, Y | |
dc.contributor.author | Di, X | |
dc.contributor.author | Shan, X | |
dc.contributor.author | Lin, C | |
dc.contributor.author | Zhang, M | |
dc.contributor.author |
Xu, X |
|
dc.contributor.author | Zhong, X | |
dc.contributor.author | Wang, J | |
dc.contributor.author | Chang, L | |
dc.contributor.author |
Halkon, B |
|
dc.contributor.author | Chen, X | |
dc.contributor.author | Cheng, F | |
dc.contributor.author | Wang, F | |
dc.date.accessioned | 2023-01-18T04:15:40Z | |
dc.date.available | 2023-01-18T04:15:40Z | |
dc.date.issued | 2022-09-14 | |
dc.identifier.citation | Nano Lett, 2022, 22, (17), pp. 7136-7143 | |
dc.identifier.issn | 1530-6984 | |
dc.identifier.issn | 1530-6992 | |
dc.identifier.uri | http://hdl.handle.net/10453/165106 | |
dc.description.abstract | Single-beam super-resolution microscopy, also known as superlinear microscopy, exploits the nonlinear response of fluorescent probes in confocal microscopy. The technique requires no complex purpose-built system, light field modulation, or beam shaping. Here, we present a strategy to enhance this technique's spatial resolution by modulating excitation intensity during image acquisition. This modulation induces dynamic optical nonlinearity in upconversion nanoparticles (UCNPs), resulting in variations of nonlinear fluorescence response in the obtained images. The higher orders of fluorescence response can be extracted with a proposed weighted finite difference imaging algorithm from raw fluorescence images to generate an image with higher resolution than superlinear microscopy images. We apply this approach to resolve single nanoparticles in a large area, improving the resolution to 132 nm. This work suggests a new scope for the development of dynamic nonlinear fluorescent probes in super-resolution nanoscopy. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | AMER CHEMICAL SOC | |
dc.relation | http://purl.org/au-research/grants/arc/DE200100074 | |
dc.relation.ispartof | Nano Lett | |
dc.relation.isbasedon | 10.1021/acs.nanolett.2c02269 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject.classification | Nanoscience & Nanotechnology | |
dc.subject.mesh | Algorithms | |
dc.subject.mesh | Fluorescent Dyes | |
dc.subject.mesh | Microscopy, Confocal | |
dc.subject.mesh | Nanoparticles | |
dc.subject.mesh | Fluorescent Dyes | |
dc.subject.mesh | Microscopy, Confocal | |
dc.subject.mesh | Algorithms | |
dc.subject.mesh | Nanoparticles | |
dc.title | Exploiting Dynamic Nonlinearity in Upconversion Nanoparticles for Super-Resolution Imaging. | |
dc.type | Journal Article | |
utslib.citation.volume | 22 | |
utslib.location.activity | United States | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Mechanical and Mechatronic Engineering | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Electrical and Data Engineering | |
pubs.organisational-group | /University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices | |
utslib.copyright.status | closed_access | * |
dc.date.updated | 2023-01-18T04:15:39Z | |
pubs.issue | 17 | |
pubs.publication-status | Published | |
pubs.volume | 22 | |
utslib.citation.issue | 17 |
Abstract:
Single-beam super-resolution microscopy, also known as superlinear microscopy, exploits the nonlinear response of fluorescent probes in confocal microscopy. The technique requires no complex purpose-built system, light field modulation, or beam shaping. Here, we present a strategy to enhance this technique's spatial resolution by modulating excitation intensity during image acquisition. This modulation induces dynamic optical nonlinearity in upconversion nanoparticles (UCNPs), resulting in variations of nonlinear fluorescence response in the obtained images. The higher orders of fluorescence response can be extracted with a proposed weighted finite difference imaging algorithm from raw fluorescence images to generate an image with higher resolution than superlinear microscopy images. We apply this approach to resolve single nanoparticles in a large area, improving the resolution to 132 nm. This work suggests a new scope for the development of dynamic nonlinear fluorescent probes in super-resolution nanoscopy.
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