Self-assembled microtissues loaded with osteogenic MSCs for in vivo bone regeneration

Li, H; He, Z; Li, W; Li, JJ; Lin, J; Xing, D

Self-assembled microtissues loaded with osteogenic MSCs for in vivo bone regeneration

Li, H He, Z Li, W Li, JJ Lin, J Xing, D

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Publisher:: Frontiers Media SA
Publication Type:: Journal Article
Citation:: Frontiers in Bioengineering and Biotechnology, 2022, 10, pp. 1069804
Issue Date:: 2022-12-12

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Field	Value	Language
dc.contributor.author	Li, H
dc.contributor.author	He, Z
dc.contributor.author	Li, W
dc.contributor.author	Li, JJ
dc.contributor.author	Lin, J
dc.contributor.author	Xing, D
dc.date.accessioned	2023-01-18T05:16:43Z
dc.date.available	2023-01-18T05:16:43Z
dc.date.issued	2022-12-12
dc.identifier.citation	Frontiers in Bioengineering and Biotechnology, 2022, 10, pp. 1069804
dc.identifier.issn	2296-4185
dc.identifier.issn	2296-4185
dc.identifier.uri	http://hdl.handle.net/10453/165123
dc.description.abstract	<jats:p>Bone regeneration strategies based on mesenchymal stem cell (MSC) therapy have received widespread attention. Although MSC incorporation into bone scaffolds can help with the repair process, a large number of studies demonstrate variable effects of MSCs with some noting that the inclusion of MSCs does not provide better outcomes compared to unseeded scaffolds. This may in part be related to low cell survival following implantation and/or limited ability to continue with osteogenic differentiation for pre-differentiated cells. In this study, we incorporated MSCs into gelatin microcryogels to form microtissues, and subjected these microtissues to osteogenic induction. We then mixed as-formed microtissues with those subjected to 6 days of osteogenic induction in different ratios, and investigated their ability to induce <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic> osteogenesis during self-assembly. Using a full-thickness rat calvarial defect model, we found that undifferentiated and osteogenically induced microtissues mixed in a ratio of 2:1 produced the best outcomes of bone regeneration. This provides a new, customizable cell-based therapeutic strategy for <jats:italic>in vivo</jats:italic> repair of bone defects.</jats:p>
dc.language	en
dc.publisher	Frontiers Media SA
dc.relation.ispartof	Frontiers in Bioengineering and Biotechnology
dc.relation.isbasedon	10.3389/fbioe.2022.1069804
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0699 Other Biological Sciences, 0903 Biomedical Engineering, 1004 Medical Biotechnology
dc.title	Self-assembled microtissues loaded with osteogenic MSCs for in vivo bone regeneration
dc.type	Journal Article
utslib.citation.volume	10
utslib.for	0699 Other Biological Sciences
utslib.for	0903 Biomedical Engineering
utslib.for	1004 Medical Biotechnology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	open_access	*
dc.date.updated	2023-01-18T05:16:40Z
pubs.publication-status	Published online
pubs.volume	10

Abstract:

Bone regeneration strategies based on mesenchymal stem cell (MSC) therapy have received widespread attention. Although MSC incorporation into bone scaffolds can help with the repair process, a large number of studies demonstrate variable effects of MSCs with some noting that the inclusion of MSCs does not provide better outcomes compared to unseeded scaffolds. This may in part be related to low cell survival following implantation and/or limited ability to continue with osteogenic differentiation for pre-differentiated cells. In this study, we incorporated MSCs into gelatin microcryogels to form microtissues, and subjected these microtissues to osteogenic induction. We then mixed as-formed microtissues with those subjected to 6 days of osteogenic induction in different ratios, and investigated their ability to induce in vitro and in vivo osteogenesis during self-assembly. Using a full-thickness rat calvarial defect model, we found that undifferentiated and osteogenically induced microtissues mixed in a ratio of 2:1 produced the best outcomes of bone regeneration. This provides a new, customizable cell-based therapeutic strategy for in vivo repair of bone defects.

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http://hdl.handle.net/10453/165123