Synergistic effect of nanomaterials and BMP-2 signalling in inducing osteogenic differentiation of adipose tissue-derived mesenchymal stem cells.

Lu, Z; Roohani-Esfahani, S-I; Li, J; Zreiqat, H

Synergistic effect of nanomaterials and BMP-2 signalling in inducing osteogenic differentiation of adipose tissue-derived mesenchymal stem cells.

Lu, Z Roohani-Esfahani, S-I Li, J

Zreiqat, H

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Publisher:: ELSEVIER SCIENCE BV
Publication Type:: Journal Article
Citation:: Nanomedicine, 2015, 11, (1), pp. 219-228
Issue Date:: 2015-01

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Field	Value	Language
dc.contributor.author	Lu, Z
dc.contributor.author	Roohani-Esfahani, S-I
dc.contributor.author	Li, J https://orcid.org/0000-0002-3584-6765
dc.contributor.author	Zreiqat, H
dc.date.accessioned	2023-01-18T05:33:46Z
dc.date.available	2014-09-15
dc.date.available	2023-01-18T05:33:46Z
dc.date.issued	2015-01
dc.identifier.citation	Nanomedicine, 2015, 11, (1), pp. 219-228
dc.identifier.issn	1549-9634
dc.identifier.issn	1549-9642
dc.identifier.uri	http://hdl.handle.net/10453/165140
dc.description.abstract	The lack of complete understanding in the signalling pathways that control the osteogenic differentiation of mesenchymal stem cells hinders their clinical application in the reconstruction of large bone defects and non-union bone fractures. The aim of this study is to gain insight into the interactions of bone morphogenetic protein-2 (BMP-2) and bone biomimetic scaffolds in directing osteogenic differentiation of adipose tissue-derived mesenchymal stem cells (ASCs) and the underlying signalling pathways involved. We demonstrated that bioactive glass nanoparticles (nBG) incorporated polycaprolactone (PCL) coating on hydroxyapatite/β-tricalcium phosphate (HA/TCP) scaffold exerted a synergistic effect with 3days of BMP-2 treatment in promoting osteogenic gene expression levels (Runx-2, collagen I, osteopontin and bone sialoprotein) and alkaline phosphatase activity in ASCs. Furthermore, we revealed that the synergistic effect was mediated through a mechanism of activating β1-integrin and induction of Wnt-3a autocrine signalling pathways by nBG incorporated scaffold.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ELSEVIER SCIENCE BV
dc.relation.ispartof	Nanomedicine
dc.relation.isbasedon	10.1016/j.nano.2014.09.008
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences, 06 Biological Sciences, 10 Technology
dc.subject.classification	Nanoscience & Nanotechnology
dc.subject.mesh	Adipose Tissue
dc.subject.mesh	Biomimetics
dc.subject.mesh	Bone Morphogenetic Protein 2
dc.subject.mesh	Calcium Phosphates
dc.subject.mesh	Cell Differentiation
dc.subject.mesh	Cell Survival
dc.subject.mesh	Collagen Type I
dc.subject.mesh	Core Binding Factor Alpha 1 Subunit
dc.subject.mesh	Durapatite
dc.subject.mesh	Gene Expression Profiling
dc.subject.mesh	Glass
dc.subject.mesh	Humans
dc.subject.mesh	Integrin beta1
dc.subject.mesh	Integrin-Binding Sialoprotein
dc.subject.mesh	Mesenchymal Stem Cells
dc.subject.mesh	Microscopy, Electron, Scanning
dc.subject.mesh	Nanomedicine
dc.subject.mesh	Nanostructures
dc.subject.mesh	Osteoblasts
dc.subject.mesh	Osteogenesis
dc.subject.mesh	Osteopontin
dc.subject.mesh	Polyesters
dc.subject.mesh	Signal Transduction
dc.subject.mesh	Wnt3A Protein
dc.subject.mesh	Adipose Tissue
dc.subject.mesh	Osteoblasts
dc.subject.mesh	Mesenchymal Stem Cells
dc.subject.mesh	Humans
dc.subject.mesh	Calcium Phosphates
dc.subject.mesh	Durapatite
dc.subject.mesh	Collagen Type I
dc.subject.mesh	Glass
dc.subject.mesh	Polyesters
dc.subject.mesh	Microscopy, Electron, Scanning
dc.subject.mesh	Gene Expression Profiling
dc.subject.mesh	Biomimetics
dc.subject.mesh	Signal Transduction
dc.subject.mesh	Cell Differentiation
dc.subject.mesh	Cell Survival
dc.subject.mesh	Osteogenesis
dc.subject.mesh	Nanostructures
dc.subject.mesh	Core Binding Factor Alpha 1 Subunit
dc.subject.mesh	Nanomedicine
dc.subject.mesh	Osteopontin
dc.subject.mesh	Bone Morphogenetic Protein 2
dc.subject.mesh	Integrin-Binding Sialoprotein
dc.subject.mesh	Wnt3A Protein
dc.subject.mesh	Integrin beta1
dc.title	Synergistic effect of nanomaterials and BMP-2 signalling in inducing osteogenic differentiation of adipose tissue-derived mesenchymal stem cells.
dc.type	Journal Article
utslib.citation.volume	11
utslib.location.activity	United States
utslib.for	03 Chemical Sciences
utslib.for	06 Biological Sciences
utslib.for	10 Technology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2023-01-18T05:33:45Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	11
utslib.citation.issue	1

Abstract:

The lack of complete understanding in the signalling pathways that control the osteogenic differentiation of mesenchymal stem cells hinders their clinical application in the reconstruction of large bone defects and non-union bone fractures. The aim of this study is to gain insight into the interactions of bone morphogenetic protein-2 (BMP-2) and bone biomimetic scaffolds in directing osteogenic differentiation of adipose tissue-derived mesenchymal stem cells (ASCs) and the underlying signalling pathways involved. We demonstrated that bioactive glass nanoparticles (nBG) incorporated polycaprolactone (PCL) coating on hydroxyapatite/β-tricalcium phosphate (HA/TCP) scaffold exerted a synergistic effect with 3days of BMP-2 treatment in promoting osteogenic gene expression levels (Runx-2, collagen I, osteopontin and bone sialoprotein) and alkaline phosphatase activity in ASCs. Furthermore, we revealed that the synergistic effect was mediated through a mechanism of activating β1-integrin and induction of Wnt-3a autocrine signalling pathways by nBG incorporated scaffold.

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http://hdl.handle.net/10453/165140