Zigzag microchannel for rigid inertial separation and enrichment (Z-RISE) of cells and particles.

Razavi Bazaz, S; Mihandust, A; Salomon, R; Joushani, HAN; Li, W; A Amiri, H; Mirakhorli, F; Zhand, S; Shrestha, J; Miansari, M; Thierry, B; Jin, D; Ebrahimi Warkiani, M

Zigzag microchannel for rigid inertial separation and enrichment (Z-RISE) of cells and particles.

Razavi Bazaz, S

Mihandust, A Salomon, R Joushani, HAN Li, W A Amiri, H Mirakhorli, F Zhand, S Shrestha, J

Miansari, M Thierry, B Jin, D

Ebrahimi Warkiani, M

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Publisher:: ROYAL SOC CHEMISTRY
Publication Type:: Journal Article
Citation:: Lab Chip, 2022, 22, (21), pp. 4093-4109
Issue Date:: 2022-10-25

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Field	Value	Language
dc.contributor.author	Razavi Bazaz, S https://orcid.org/0000-0002-6419-3361
dc.contributor.author	Mihandust, A
dc.contributor.author	Salomon, R
dc.contributor.author	Joushani, HAN
dc.contributor.author	Li, W
dc.contributor.author	A Amiri, H
dc.contributor.author	Mirakhorli, F
dc.contributor.author	Zhand, S
dc.contributor.author	Shrestha, J https://orcid.org/0000-0003-3692-6676
dc.contributor.author	Miansari, M
dc.contributor.author	Thierry, B
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666
dc.contributor.author	Ebrahimi Warkiani, M
dc.date.accessioned	2023-03-14T00:07:25Z
dc.date.available	2023-03-14T00:07:25Z
dc.date.issued	2022-10-25
dc.identifier.citation	Lab Chip, 2022, 22, (21), pp. 4093-4109
dc.identifier.issn	1473-0197
dc.identifier.issn	1473-0189
dc.identifier.uri	http://hdl.handle.net/10453/167276
dc.description.abstract	Separation and enrichment of target cells prior to downstream analyses is an essential pre-treatment step in many biomedical and clinical assays. Separation techniques utilizing simple, cost-effective, and user-friendly devices are highly desirable, both in the lab and at the point of need. Passive microfluidic approaches, especially inertial microfluidics, fit this brief perfectly and are highly desired. Using an optimized additive manufacturing technique, we developed a zigzag microchannel for rigid inertial separation and enrichment, hereafter referred to as Z-RISE. We empirically showed that the Z-RISE device outperforms equivalent devices based on curvilinear (sinusoidal), asymmetric curvilinear, zigzag with round corners, or square-wave formats and modelled this behavior to gain a better understanding of the physics underpinning the improved focusing and separation performance. The comparison between rigid and soft zigzag microchannels reveals that channel rigidity significantly affects and enhances the focusing performance of the microchannel. Compared to other serpentine microchannels, zigzag microfluidics demonstrates superior separation and purity efficiency due to the sudden channel cross-section expansion at the corners. Within Z-RISE, particles are aligned in either double-side or single-line focusing positions. The transition of particles from a double-focusing line to a single focusing line introduced a new phenomenon referred to as the plus focusing position. We experimentally demonstrated that Z-RISE could enrich leukocytes and their subtypes from diluted and RBC lysed blood while depleting dead cells, debris, and RBCs. Z-RISE was also shown to yield outstanding particle or cell concentration with a concentration efficiency of more than 99.99%. Our data support the great potential of Z-RISE for applications that involve particle and cell manipulations and pave the way for commercialization perspective in the near future.
dc.format	Electronic
dc.language	eng
dc.publisher	ROYAL SOC CHEMISTRY
dc.relation	http://purl.org/au-research/grants/arc/DP200101860
dc.relation.ispartof	Lab Chip
dc.relation.isbasedon	10.1039/d2lc00290f
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences, 09 Engineering
dc.subject.classification	Analytical Chemistry
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Microfluidics
dc.subject.mesh	Leukocytes
dc.subject.mesh	Erythrocytes
dc.subject.mesh	Erythrocyte Count
dc.subject.mesh	Cell Separation
dc.subject.mesh	Erythrocytes
dc.subject.mesh	Leukocytes
dc.subject.mesh	Erythrocyte Count
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Cell Separation
dc.subject.mesh	Microfluidics
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Microfluidics
dc.subject.mesh	Leukocytes
dc.subject.mesh	Erythrocytes
dc.subject.mesh	Erythrocyte Count
dc.subject.mesh	Cell Separation
dc.title	Zigzag microchannel for rigid inertial separation and enrichment (Z-RISE) of cells and particles.
dc.type	Journal Article
utslib.citation.volume	22
utslib.location.activity	England
utslib.for	03 Chemical Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2023-03-14T00:07:17Z
pubs.issue	21
pubs.publication-status	Published online
pubs.volume	22
utslib.citation.issue	21

Abstract:

Separation and enrichment of target cells prior to downstream analyses is an essential pre-treatment step in many biomedical and clinical assays. Separation techniques utilizing simple, cost-effective, and user-friendly devices are highly desirable, both in the lab and at the point of need. Passive microfluidic approaches, especially inertial microfluidics, fit this brief perfectly and are highly desired. Using an optimized additive manufacturing technique, we developed a zigzag microchannel for rigid inertial separation and enrichment, hereafter referred to as Z-RISE. We empirically showed that the Z-RISE device outperforms equivalent devices based on curvilinear (sinusoidal), asymmetric curvilinear, zigzag with round corners, or square-wave formats and modelled this behavior to gain a better understanding of the physics underpinning the improved focusing and separation performance. The comparison between rigid and soft zigzag microchannels reveals that channel rigidity significantly affects and enhances the focusing performance of the microchannel. Compared to other serpentine microchannels, zigzag microfluidics demonstrates superior separation and purity efficiency due to the sudden channel cross-section expansion at the corners. Within Z-RISE, particles are aligned in either double-side or single-line focusing positions. The transition of particles from a double-focusing line to a single focusing line introduced a new phenomenon referred to as the plus focusing position. We experimentally demonstrated that Z-RISE could enrich leukocytes and their subtypes from diluted and RBC lysed blood while depleting dead cells, debris, and RBCs. Z-RISE was also shown to yield outstanding particle or cell concentration with a concentration efficiency of more than 99.99%. Our data support the great potential of Z-RISE for applications that involve particle and cell manipulations and pave the way for commercialization perspective in the near future.

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http://hdl.handle.net/10453/167276