Relationship between Serum Testosterone and Fracture Risk in Men: A Comparison of RIA and LC-MS/MS.

Tran, TS; Center, JR; Seibel, MJ; Eisman, JA; Kushnir, MM; Rockwood, AL; Nguyen, TV

Relationship between Serum Testosterone and Fracture Risk in Men: A Comparison of RIA and LC-MS/MS.

Tran, TS Center, JR Seibel, MJ Eisman, JA Kushnir, MM Rockwood, AL Nguyen, TV

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Publisher:: AMER ASSOC CLINICAL CHEMISTRY
Publication Type:: Journal Article
Citation:: Clin Chem, 2015, 61, (9), pp. 1182-1190
Issue Date:: 2015-09

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Field	Value	Language
dc.contributor.author	Tran, TS
dc.contributor.author	Center, JR
dc.contributor.author	Seibel, MJ
dc.contributor.author	Eisman, JA
dc.contributor.author	Kushnir, MM
dc.contributor.author	Rockwood, AL
dc.contributor.author	Nguyen, TV
dc.date.accessioned	2023-03-19T09:12:32Z
dc.date.available	2015-06-09
dc.date.available	2023-03-19T09:12:32Z
dc.date.issued	2015-09
dc.identifier.citation	Clin Chem, 2015, 61, (9), pp. 1182-1190
dc.identifier.issn	0009-9147
dc.identifier.issn	1530-8561
dc.identifier.uri	http://hdl.handle.net/10453/167557
dc.description.abstract	BACKGROUND: Serum testosterone can be measured by LC-MS/MS and RIA. We investigated whether the testosterone-fracture relationship was affected by the method of measurement. METHODS: We measured total testosterone (TT) by LC-MS/MS (TTLC-MS/MS) and RIA (TTRIA) in serum samples collected from 602 men whose incident fractures had been continuously ascertained by x-ray reports from 1989 to 2010. We measured bone mineral density (BMD) by dual-energy x-ray absorptiometry. The association between TT and fracture risk was assessed by the Cox proportional hazards model, taking into account the effect of age and BMD. RESULTS: Mean TTLC-MS/MS was higher than TTRIA by 27 ng/dL (95% CI 13-41). The concordance correlation coefficient between TTLC-MS/MS and TTRIA was 0.72 (95% CI 0.68-0.76). The Deming regression equation linking the 2 measurements was ln(TTLC-MS/MS + 10) = 0.87 + 0.87 × ln(TTRIA + 10). The hazard ratio of fracture per SD decrease in TT was 1.32 (95% CI 1.12-1.54) for TTLC-MS/MS and 1.23 (1.06-1.43) for TTRIA. The correlation between predicted probabilities of fracture by TTLC-MS/MS and TTRIA was r = 0.96, with the mean difference being 0.01% (95% CI -6.1% to 6.2%). Slightly more patients were classified as having hypogonadism if TTRIA was used (29% vs 26%). CONCLUSIONS: The concordance between LC-MS/MS and RIA in the measurement of serum TT was moderate. Moreover, the magnitude of association between testosterone and fracture risk in older men was largely unaffected by the method of measurement.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER ASSOC CLINICAL CHEMISTRY
dc.relation.ispartof	Clin Chem
dc.relation.isbasedon	10.1373/clinchem.2015.242339
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	1004 Medical Biotechnology, 1101 Medical Biochemistry and Metabolomics, 1103 Clinical Sciences
dc.subject.classification	General Clinical Medicine
dc.subject.mesh	Aged
dc.subject.mesh	Bone Density
dc.subject.mesh	Chromatography, Liquid
dc.subject.mesh	Fractures, Bone
dc.subject.mesh	Humans
dc.subject.mesh	Male
dc.subject.mesh	Proportional Hazards Models
dc.subject.mesh	Risk Factors
dc.subject.mesh	Tandem Mass Spectrometry
dc.subject.mesh	Testosterone
dc.subject.mesh	Humans
dc.subject.mesh	Testosterone
dc.subject.mesh	Chromatography, Liquid
dc.subject.mesh	Proportional Hazards Models
dc.subject.mesh	Risk Factors
dc.subject.mesh	Bone Density
dc.subject.mesh	Aged
dc.subject.mesh	Male
dc.subject.mesh	Fractures, Bone
dc.subject.mesh	Tandem Mass Spectrometry
dc.subject.mesh	Aged
dc.subject.mesh	Bone Density
dc.subject.mesh	Chromatography, Liquid
dc.subject.mesh	Fractures, Bone
dc.subject.mesh	Humans
dc.subject.mesh	Male
dc.subject.mesh	Proportional Hazards Models
dc.subject.mesh	Risk Factors
dc.subject.mesh	Tandem Mass Spectrometry
dc.subject.mesh	Testosterone
dc.title	Relationship between Serum Testosterone and Fracture Risk in Men: A Comparison of RIA and LC-MS/MS.
dc.type	Journal Article
utslib.citation.volume	61
utslib.location.activity	England
utslib.for	1101 Medical Biochemistry and Metabolomics
utslib.for	1004 Medical Biotechnology
utslib.for	1101 Medical Biochemistry and Metabolomics
utslib.for	1103 Clinical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2023-03-19T09:12:31Z
pubs.issue	9
pubs.publication-status	Published
pubs.volume	61
utslib.citation.issue	9

Abstract:

BACKGROUND: Serum testosterone can be measured by LC-MS/MS and RIA. We investigated whether the testosterone-fracture relationship was affected by the method of measurement. METHODS: We measured total testosterone (TT) by LC-MS/MS (TTLC-MS/MS) and RIA (TTRIA) in serum samples collected from 602 men whose incident fractures had been continuously ascertained by x-ray reports from 1989 to 2010. We measured bone mineral density (BMD) by dual-energy x-ray absorptiometry. The association between TT and fracture risk was assessed by the Cox proportional hazards model, taking into account the effect of age and BMD. RESULTS: Mean TTLC-MS/MS was higher than TTRIA by 27 ng/dL (95% CI 13-41). The concordance correlation coefficient between TTLC-MS/MS and TTRIA was 0.72 (95% CI 0.68-0.76). The Deming regression equation linking the 2 measurements was ln(TTLC-MS/MS + 10) = 0.87 + 0.87 × ln(TTRIA + 10). The hazard ratio of fracture per SD decrease in TT was 1.32 (95% CI 1.12-1.54) for TTLC-MS/MS and 1.23 (1.06-1.43) for TTRIA. The correlation between predicted probabilities of fracture by TTLC-MS/MS and TTRIA was r = 0.96, with the mean difference being 0.01% (95% CI -6.1% to 6.2%). Slightly more patients were classified as having hypogonadism if TTRIA was used (29% vs 26%). CONCLUSIONS: The concordance between LC-MS/MS and RIA in the measurement of serum TT was moderate. Moreover, the magnitude of association between testosterone and fracture risk in older men was largely unaffected by the method of measurement.

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http://hdl.handle.net/10453/167557