Giardia purification from fecal samples using rigid spiral inertial microfluidics

Ding, L; Razavi Bazaz, S; Hall, T; Vesey, G; Ebrahimi Warkiani, M

Giardia purification from fecal samples using rigid spiral inertial microfluidics

Ding, L Razavi Bazaz, S

Hall, T Vesey, G Ebrahimi Warkiani, M

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Publisher:: AIP Publishing
Publication Type:: Journal Article
Citation:: Biomicrofluidics, 2022, 16, (1)
Issue Date:: 2022-01-01

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Field	Value	Language
dc.contributor.author	Ding, L
dc.contributor.author	Razavi Bazaz, S https://orcid.org/0000-0002-6419-3361
dc.contributor.author	Hall, T
dc.contributor.author	Vesey, G
dc.contributor.author	Ebrahimi Warkiani, M https://orcid.org/0000-0002-4184-1944
dc.date.accessioned	2023-04-06T00:11:42Z
dc.date.available	2023-04-06T00:11:42Z
dc.date.issued	2022-01-01
dc.identifier.citation	Biomicrofluidics, 2022, 16, (1)
dc.identifier.issn	1932-1058
dc.identifier.issn	1932-1058
dc.identifier.uri	http://hdl.handle.net/10453/169226
dc.description.abstract	Giardia is one of the most common waterborne pathogens causing around 200 × 106 diarrheal infections annually. It is of great interest to microbiological research as it is among the oldest known eukaryotic cells. Purifying Giardia from fecal samples for both research and diagnostic purposes presents one of the most difficult challenges. Traditional purification methods rely on density gradient centrifugation, membrane-based filtration, and sedimentation methods, which suffer from low recovery rates, high costs, and poor efficiency. Here, we report on the use of microfluidics to purify Giardia cysts from mouse feces. We propose a rigid spiral microfluidic device with a trapezoidal cross section to effectively separate Giardia from surrounding debris. Our characterizations reveal that the recovery rate is concentration-dependent, and our proposed device can achieve recovery rates as high as 75% with 0.75 ml/min throughput. Moreover, this device can purify Giardia from extremely turbid samples to a level where cysts are visually distinguishable with just one round of purification. This highly scalable and versatile 3D printed microfluidic device is then capable of further purifying or enhancing the recovery rate of the samples by recirculation. This device also has the potential to purify other gastrointestinal pathogens of similar size, and throughput can be significantly increased by parallelization.
dc.language	English
dc.publisher	AIP Publishing
dc.relation	http://purl.org/au-research/grants/arc/DP170103704
dc.relation	http://purl.org/au-research/grants/arc/DP180103003
dc.relation	http://purl.org/au-research/grants/nhmrc/1143377
dc.relation.ispartof	Biomicrofluidics
dc.relation.isbasedon	10.1063/5.0069406
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in “Giardia purification from fecal samples using rigid spiral inertial microfluidics. Biomicrofluidics 16, 014105 (2022) and may be found at https://doi.org/10.1063/5.0069406”
dc.subject	0203 Classical Physics, 0915 Interdisciplinary Engineering, 1007 Nanotechnology
dc.subject.classification	Nanoscience & Nanotechnology
dc.title	Giardia purification from fecal samples using rigid spiral inertial microfluidics
dc.type	Journal Article
utslib.citation.volume	16
utslib.for	0203 Classical Physics
utslib.for	0915 Interdisciplinary Engineering
utslib.for	1007 Nanotechnology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
dc.date.updated	2023-04-06T00:11:41Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	16
utslib.citation.issue	1

Abstract:

Giardia is one of the most common waterborne pathogens causing around 200 × 106 diarrheal infections annually. It is of great interest to microbiological research as it is among the oldest known eukaryotic cells. Purifying Giardia from fecal samples for both research and diagnostic purposes presents one of the most difficult challenges. Traditional purification methods rely on density gradient centrifugation, membrane-based filtration, and sedimentation methods, which suffer from low recovery rates, high costs, and poor efficiency. Here, we report on the use of microfluidics to purify Giardia cysts from mouse feces. We propose a rigid spiral microfluidic device with a trapezoidal cross section to effectively separate Giardia from surrounding debris. Our characterizations reveal that the recovery rate is concentration-dependent, and our proposed device can achieve recovery rates as high as 75% with 0.75 ml/min throughput. Moreover, this device can purify Giardia from extremely turbid samples to a level where cysts are visually distinguishable with just one round of purification. This highly scalable and versatile 3D printed microfluidic device is then capable of further purifying or enhancing the recovery rate of the samples by recirculation. This device also has the potential to purify other gastrointestinal pathogens of similar size, and throughput can be significantly increased by parallelization.

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http://hdl.handle.net/10453/169226