Rapid separation of bacteria from primary nasal samples using inertial microfluidics.

Shrestha, J; Razavi Bazaz, S; Ding, L; Vasilescu, S; Idrees, S; Söderström, B; Hansbro, PM; Ghadiri, M; Ebrahimi Warkiani, M

Rapid separation of bacteria from primary nasal samples using inertial microfluidics.

Shrestha, J

Razavi Bazaz, S

Ding, L Vasilescu, S Idrees, S Söderström, B Hansbro, PM Ghadiri, M Ebrahimi Warkiani, M

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Publisher:: ROYAL SOC CHEMISTRY
Publication Type:: Journal Article
Citation:: Lab Chip, 2022, 23, (1), pp. 146-156
Issue Date:: 2022-12-20

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Field	Value	Language
dc.contributor.author	Shrestha, J https://orcid.org/0000-0003-3692-6676
dc.contributor.author	Razavi Bazaz, S https://orcid.org/0000-0002-6419-3361
dc.contributor.author	Ding, L
dc.contributor.author	Vasilescu, S
dc.contributor.author	Idrees, S
dc.contributor.author	Söderström, B
dc.contributor.author	Hansbro, PM
dc.contributor.author	Ghadiri, M
dc.contributor.author	Ebrahimi Warkiani, M https://orcid.org/0000-0002-4184-1944
dc.date.accessioned	2023-04-26T04:41:22Z
dc.date.available	2023-04-26T04:41:22Z
dc.date.issued	2022-12-20
dc.identifier.citation	Lab Chip, 2022, 23, (1), pp. 146-156
dc.identifier.issn	1473-0197
dc.identifier.issn	1473-0189
dc.identifier.uri	http://hdl.handle.net/10453/170114
dc.description.abstract	Microbial populations play a crucial role in human health and the development of many diseases. These diseases often arise from the explosive proliferation of opportunistic bacteria, such as those in the nasal cavity. Recently, there have been increases in the prevalence of these opportunistic pathogens displaying antibiotic resistance. Thus, the study of the nasal microbiota and its bacterial diversity is critical in understanding pathogenesis and developing microbial-based therapies for well-known and emerging diseases. However, the isolation and analysis of these populations for clinical study complicates the already challenging task of identifying and profiling potentially harmful bacteria. Existing methods are limited by low sample throughput, expensive labeling, and low recovery of bacteria with ineffective removal of cells and debris. In this study, we propose a novel microfluidic channel with a zigzag configuration for enhanced isolation and detection of bacteria from human clinical nasal swabs. This microfluidic zigzag channel separates the bacteria from epithelial cells and debris by size differential focusing. As such, pure bacterial cell fractions devoid of large contaminating debris or epithelial cells are obtained. DNA sequencing performed on the separated bacteria defines the diversity and species present. This novel method of bacterial separation is simple, robust, rapid, and cost-effective and has the potential to be used for the rapid identification of bacterial cell populations from clinical samples.
dc.format	Electronic
dc.language	eng
dc.publisher	ROYAL SOC CHEMISTRY
dc.relation	http://purl.org/au-research/grants/nhmrc/1175134
dc.relation.ispartof	Lab Chip
dc.relation.isbasedon	10.1039/d2lc00794k
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences, 09 Engineering
dc.subject.classification	Analytical Chemistry
dc.subject.mesh	Humans
dc.subject.mesh	Microfluidics
dc.subject.mesh	Bacteria
dc.subject.mesh	Sequence Analysis, DNA
dc.subject.mesh	Cell Separation
dc.subject.mesh	Humans
dc.subject.mesh	Bacteria
dc.subject.mesh	Cell Separation
dc.subject.mesh	Sequence Analysis, DNA
dc.subject.mesh	Microfluidics
dc.subject.mesh	Humans
dc.subject.mesh	Microfluidics
dc.subject.mesh	Bacteria
dc.subject.mesh	Sequence Analysis, DNA
dc.subject.mesh	Cell Separation
dc.title	Rapid separation of bacteria from primary nasal samples using inertial microfluidics.
dc.type	Journal Article
utslib.citation.volume	23
utslib.location.activity	England
utslib.for	03 Chemical Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
pubs.organisational-group	/University of Technology Sydney/Strength - CFI - Centre for Inflammation
utslib.copyright.status	closed_access	*
dc.date.updated	2023-04-26T04:41:21Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	23
utslib.citation.issue	1

Abstract:

Microbial populations play a crucial role in human health and the development of many diseases. These diseases often arise from the explosive proliferation of opportunistic bacteria, such as those in the nasal cavity. Recently, there have been increases in the prevalence of these opportunistic pathogens displaying antibiotic resistance. Thus, the study of the nasal microbiota and its bacterial diversity is critical in understanding pathogenesis and developing microbial-based therapies for well-known and emerging diseases. However, the isolation and analysis of these populations for clinical study complicates the already challenging task of identifying and profiling potentially harmful bacteria. Existing methods are limited by low sample throughput, expensive labeling, and low recovery of bacteria with ineffective removal of cells and debris. In this study, we propose a novel microfluidic channel with a zigzag configuration for enhanced isolation and detection of bacteria from human clinical nasal swabs. This microfluidic zigzag channel separates the bacteria from epithelial cells and debris by size differential focusing. As such, pure bacterial cell fractions devoid of large contaminating debris or epithelial cells are obtained. DNA sequencing performed on the separated bacteria defines the diversity and species present. This novel method of bacterial separation is simple, robust, rapid, and cost-effective and has the potential to be used for the rapid identification of bacterial cell populations from clinical samples.

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http://hdl.handle.net/10453/170114