A Microfluidic Approach for Enrichment and Single-Cell Characterization of Circulating Tumor Cells from Peripheral Blood.

Radfar, P; Ding, L; Es, HA; Warkiani, ME

A Microfluidic Approach for Enrichment and Single-Cell Characterization of Circulating Tumor Cells from Peripheral Blood.

Radfar, P Ding, L Es, HA Warkiani, ME

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Publisher:: Humana Press
Publication Type:: Journal Article
Citation:: Methods in Molecular Biology, 2023, 2679, pp. 141-150
Issue Date:: 2023

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Field	Value	Language
dc.contributor.author	Radfar, P
dc.contributor.author	Ding, L
dc.contributor.author	Es, HA
dc.contributor.author	Warkiani, ME
dc.date.accessioned	2024-02-06T02:32:43Z
dc.date.available	2024-02-06T02:32:43Z
dc.date.issued	2023
dc.identifier.citation	Methods in Molecular Biology, 2023, 2679, pp. 141-150
dc.identifier.isbn	978-1-0716-3270-3
dc.identifier.issn	1064-3745
dc.identifier.issn	1940-6029
dc.identifier.uri	http://hdl.handle.net/10453/175351
dc.description.abstract	The emergence of enabling technologies for the analysis of circulating tumor cells has been shedding new lights into cancer management in the recent years. However, majority of the technologies developed suffer from excessive cost, time-consuming workflows, and reliance on specialized equipment and operators. Herein, we propose a simple workflow for the isolation and characterization of single circulating tumor cells using microfluidic devices. The entire process can be operated by a laboratory technician without relying on any microfluidic expertise and can be completed within few hours of sample collection.
dc.format	Print
dc.language	eng
dc.publisher	Humana Press
dc.relation.ispartof	Methods in Molecular Biology
dc.relation.isbasedon	10.1007/978-1-0716-3271-0_9
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.subject.classification	3101 Biochemistry and cell biology
dc.subject.classification	3404 Medicinal and biomolecular chemistry
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Cell Separation
dc.subject.mesh	Humans
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Microfluidics
dc.subject.mesh	Neoplastic Cells, Circulating
dc.subject.mesh	Workflow
dc.subject.mesh	Humans
dc.subject.mesh	Microfluidics
dc.subject.mesh	Neoplastic Cells, Circulating
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Workflow
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Cell Separation
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Humans
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Cell Separation
dc.subject.mesh	Microfluidics
dc.subject.mesh	Neoplastic Cells, Circulating
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Workflow
dc.subject.mesh	Humans
dc.subject.mesh	Microfluidics
dc.subject.mesh	Neoplastic Cells, Circulating
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Workflow
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Cell Line, Tumor
dc.subject.mesh	Cell Separation
dc.title	A Microfluidic Approach for Enrichment and Single-Cell Characterization of Circulating Tumor Cells from Peripheral Blood.
dc.type	Journal Article
utslib.citation.volume	2679
utslib.location.activity	United States
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2024-02-06T02:32:42Z
pubs.publication-status	Published
pubs.volume	2679

Abstract:

The emergence of enabling technologies for the analysis of circulating tumor cells has been shedding new lights into cancer management in the recent years. However, majority of the technologies developed suffer from excessive cost, time-consuming workflows, and reliance on specialized equipment and operators. Herein, we propose a simple workflow for the isolation and characterization of single circulating tumor cells using microfluidic devices. The entire process can be operated by a laboratory technician without relying on any microfluidic expertise and can be completed within few hours of sample collection.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/175351