Microscale geometrical modulation of PIEZO1 mediated mechanosensing through cytoskeletal redistribution.
Wang, HJ
Wang, Y
Mirjavadi, SS
Andersen, T
Moldovan, L
Vatankhah, P
Russell, B
Jin, J
Zhou, Z
Li, Q
Cox, CD
Su, QP
Ju, LA
- Publisher:
- NATURE PORTFOLIO
- Publication Type:
- Journal Article
- Citation:
- Nat Commun, 2024, 15, (1), pp. 5521
- Issue Date:
- 2024-06-29
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, HJ | |
dc.contributor.author | Wang, Y | |
dc.contributor.author | Mirjavadi, SS | |
dc.contributor.author | Andersen, T | |
dc.contributor.author | Moldovan, L | |
dc.contributor.author | Vatankhah, P | |
dc.contributor.author | Russell, B | |
dc.contributor.author | Jin, J | |
dc.contributor.author | Zhou, Z | |
dc.contributor.author | Li, Q | |
dc.contributor.author | Cox, CD | |
dc.contributor.author | Su, QP | |
dc.contributor.author | Ju, LA | |
dc.date.accessioned | 2024-08-01T04:43:23Z | |
dc.date.available | 2024-06-20 | |
dc.date.available | 2024-08-01T04:43:23Z | |
dc.date.issued | 2024-06-29 | |
dc.identifier.citation | Nat Commun, 2024, 15, (1), pp. 5521 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | http://hdl.handle.net/10453/179960 | |
dc.description.abstract | The microgeometry of the cellular microenvironment profoundly impacts cellular behaviors, yet the link between it and the ubiquitously expressed mechanosensitive ion channel PIEZO1 remains unclear. Herein, we describe a fluorescent micropipette aspiration assay that allows for simultaneous visualization of intracellular calcium dynamics and cytoskeletal architecture in real-time, under varied micropipette geometries. By integrating elastic shell finite element analysis with fluorescent lifetime imaging microscopy and employing PIEZO1-specific transgenic red blood cells and HEK cell lines, we demonstrate a direct correlation between the microscale geometry of aspiration and PIEZO1-mediated calcium signaling. We reveal that increased micropipette tip angles and physical constrictions lead to a significant reorganization of F-actin, accumulation at the aspirated cell neck, and subsequently amplify the tension stress at the dome of the cell to induce more PIEZO1's activity. Disruption of the F-actin network or inhibition of its mobility leads to a notable decline in PIEZO1 mediated calcium influx, underscoring its critical role in cellular mechanosensing amidst geometrical constraints. | |
dc.format | Electronic | |
dc.language | eng | |
dc.publisher | NATURE PORTFOLIO | |
dc.relation | http://purl.org/au-research/grants/arc/DP200101970 | |
dc.relation | http://purl.org/au-research/grants/nhmrc/1177374 | |
dc.relation.ispartof | Nat Commun | |
dc.relation.isbasedon | 10.1038/s41467-024-49833-6 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Ion Channels | |
dc.subject.mesh | Mechanotransduction, Cellular | |
dc.subject.mesh | Actins | |
dc.subject.mesh | HEK293 Cells | |
dc.subject.mesh | Cytoskeleton | |
dc.subject.mesh | Calcium | |
dc.subject.mesh | Calcium Signaling | |
dc.subject.mesh | Finite Element Analysis | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Microscopy, Fluorescence | |
dc.subject.mesh | Cytoskeleton | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Calcium | |
dc.subject.mesh | Actins | |
dc.subject.mesh | Ion Channels | |
dc.subject.mesh | Microscopy, Fluorescence | |
dc.subject.mesh | Mechanotransduction, Cellular | |
dc.subject.mesh | Calcium Signaling | |
dc.subject.mesh | Finite Element Analysis | |
dc.subject.mesh | HEK293 Cells | |
dc.title | Microscale geometrical modulation of PIEZO1 mediated mechanosensing through cytoskeletal redistribution. | |
dc.type | Journal Article | |
utslib.citation.volume | 15 | |
utslib.location.activity | England | |
pubs.organisational-group | University of Technology Sydney | |
pubs.organisational-group | University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
utslib.copyright.status | open_access | * |
dc.rights.license | This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/ | |
dc.date.updated | 2024-08-01T04:43:14Z | |
pubs.issue | 1 | |
pubs.publication-status | Published online | |
pubs.volume | 15 | |
utslib.citation.issue | 1 |
Abstract:
The microgeometry of the cellular microenvironment profoundly impacts cellular behaviors, yet the link between it and the ubiquitously expressed mechanosensitive ion channel PIEZO1 remains unclear. Herein, we describe a fluorescent micropipette aspiration assay that allows for simultaneous visualization of intracellular calcium dynamics and cytoskeletal architecture in real-time, under varied micropipette geometries. By integrating elastic shell finite element analysis with fluorescent lifetime imaging microscopy and employing PIEZO1-specific transgenic red blood cells and HEK cell lines, we demonstrate a direct correlation between the microscale geometry of aspiration and PIEZO1-mediated calcium signaling. We reveal that increased micropipette tip angles and physical constrictions lead to a significant reorganization of F-actin, accumulation at the aspirated cell neck, and subsequently amplify the tension stress at the dome of the cell to induce more PIEZO1's activity. Disruption of the F-actin network or inhibition of its mobility leads to a notable decline in PIEZO1 mediated calcium influx, underscoring its critical role in cellular mechanosensing amidst geometrical constraints.
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