Biocompatibility and proteomic profiling of DMSA-coated iron nanocubes in a human glioblastoma cell line.

Ulanova, M; Gloag, L; Kim, C-K; Bongers, A; Kim Duong, HT; Gooding, JJ; Tilley, RD; Sachdev, PS; Braidy, N

Biocompatibility and proteomic profiling of DMSA-coated iron nanocubes in a human glioblastoma cell line.

Ulanova, M Gloag, L

Kim, C-K Bongers, A Kim Duong, HT Gooding, JJ Tilley, RD Sachdev, PS Braidy, N

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Publisher:: TAYLOR & FRANCIS LTD
Publication Type:: Journal Article
Citation:: Nanomedicine (Lond), 2024, 19, (4), pp. 303-323
Issue Date:: 2024-02

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Field	Value	Language
dc.contributor.author	Ulanova, M
dc.contributor.author	Gloag, L https://orcid.org/0000-0001-7548-1521
dc.contributor.author	Kim, C-K
dc.contributor.author	Bongers, A
dc.contributor.author	Kim Duong, HT
dc.contributor.author	Gooding, JJ
dc.contributor.author	Tilley, RD
dc.contributor.author	Sachdev, PS
dc.contributor.author	Braidy, N
dc.date.accessioned	2024-11-22T02:28:40Z
dc.date.available	2024-11-22T02:28:40Z
dc.date.issued	2024-02
dc.identifier.citation	Nanomedicine (Lond), 2024, 19, (4), pp. 303-323
dc.identifier.issn	1743-5889
dc.identifier.issn	1748-6963
dc.identifier.uri	http://hdl.handle.net/10453/182056
dc.description.abstract	Background: Superparamagnetic iron core iron oxide shell nanocubes have previously shown superior performance in magnetic resonance imaging T2 contrast enhancement compared with spherical nanoparticles. Methods: Iron core iron oxide shell nanocubes were synthesized, stabilized with dimercaptosuccinic acid (DMSA-NC) and physicochemically characterized. MRI contrast enhancement and biocompatibility were assessed in vitro. Results: DMSA-NC showed a transverse relaxivity of 122.59 mM-1·s-1 Fe. Treatment with DMSA-NC did not induce cytotoxicity or oxidative stress in U-251 cells, and electron microscopy demonstrated DMSA-NC localization within endosomes and lysosomes in cells following internalization. Global proteomics revealed dysregulation of iron storage, transport, transcription and mRNA processing proteins. Conclusion: DMSA-NC is a promising T2 MRI contrast agent which, in this preliminary investigation, demonstrates favorable biocompatibility with an astrocyte cell model.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	TAYLOR & FRANCIS LTD
dc.relation.ispartof	Nanomedicine (Lond)
dc.relation.isbasedon	10.2217/nnm-2023-0304
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0306 Physical Chemistry (incl. Structural), 1004 Medical Biotechnology, 1007 Nanotechnology
dc.subject.classification	Nanoscience & Nanotechnology
dc.subject.classification	3206 Medical biotechnology
dc.subject.classification	4003 Biomedical engineering
dc.subject.classification	4018 Nanotechnology
dc.subject.mesh	Humans
dc.subject.mesh	Iron
dc.subject.mesh	Magnetite Nanoparticles
dc.subject.mesh	Glioblastoma
dc.subject.mesh	Proteomics
dc.subject.mesh	Ferric Compounds
dc.subject.mesh	Cell Line
dc.subject.mesh	Contrast Media
dc.subject.mesh	Magnetic Resonance Imaging
dc.subject.mesh	Succimer
dc.subject.mesh	Cell Line
dc.subject.mesh	Humans
dc.subject.mesh	Glioblastoma
dc.subject.mesh	Iron
dc.subject.mesh	Ferric Compounds
dc.subject.mesh	Succimer
dc.subject.mesh	Contrast Media
dc.subject.mesh	Magnetic Resonance Imaging
dc.subject.mesh	Proteomics
dc.subject.mesh	Magnetite Nanoparticles
dc.subject.mesh	Humans
dc.subject.mesh	Iron
dc.subject.mesh	Magnetite Nanoparticles
dc.subject.mesh	Glioblastoma
dc.subject.mesh	Proteomics
dc.subject.mesh	Ferric Compounds
dc.subject.mesh	Cell Line
dc.subject.mesh	Contrast Media
dc.subject.mesh	Magnetic Resonance Imaging
dc.subject.mesh	Succimer
dc.title	Biocompatibility and proteomic profiling of DMSA-coated iron nanocubes in a human glioblastoma cell line.
dc.type	Journal Article
utslib.citation.volume	19
utslib.location.activity	England
utslib.for	0306 Physical Chemistry (incl. Structural)
utslib.for	1004 Medical Biotechnology
utslib.for	1007 Nanotechnology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2024-11-22T02:28:35Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	19
utslib.citation.issue	4

Abstract:

Background: Superparamagnetic iron core iron oxide shell nanocubes have previously shown superior performance in magnetic resonance imaging T2 contrast enhancement compared with spherical nanoparticles. Methods: Iron core iron oxide shell nanocubes were synthesized, stabilized with dimercaptosuccinic acid (DMSA-NC) and physicochemically characterized. MRI contrast enhancement and biocompatibility were assessed in vitro. Results: DMSA-NC showed a transverse relaxivity of 122.59 mM-1·s-1 Fe. Treatment with DMSA-NC did not induce cytotoxicity or oxidative stress in U-251 cells, and electron microscopy demonstrated DMSA-NC localization within endosomes and lysosomes in cells following internalization. Global proteomics revealed dysregulation of iron storage, transport, transcription and mRNA processing proteins. Conclusion: DMSA-NC is a promising T2 MRI contrast agent which, in this preliminary investigation, demonstrates favorable biocompatibility with an astrocyte cell model.

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http://hdl.handle.net/10453/182056