Zirconium ions up-regulate the BMP/SMAD signaling pathway and promote the proliferation and differentiation of human osteoblasts.

Chen, Y; Roohani-Esfahani, S-I; Lu, Z; Zreiqat, H; Dunstan, CR

Zirconium ions up-regulate the BMP/SMAD signaling pathway and promote the proliferation and differentiation of human osteoblasts.

Chen, Y Roohani-Esfahani, S-I Lu, Z Zreiqat, H Dunstan, CR

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Publisher:: PUBLIC LIBRARY SCIENCE
Publication Type:: Journal Article
Citation:: PLoS One, 2015, 10, (1), pp. e0113426
Issue Date:: 2015

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Field	Value	Language
dc.contributor.author	Chen, Y
dc.contributor.author	Roohani-Esfahani, S-I
dc.contributor.author	Lu, Z
dc.contributor.author	Zreiqat, H
dc.contributor.author	Dunstan, CR
dc.contributor.editor	Marie, PJ
dc.date.accessioned	2025-01-15T20:44:13Z
dc.date.available	2014-10-27
dc.date.available	2025-01-15T20:44:13Z
dc.date.issued	2015
dc.identifier.citation	PLoS One, 2015, 10, (1), pp. e0113426
dc.identifier.issn	1932-6203
dc.identifier.issn	1932-6203
dc.identifier.uri	http://hdl.handle.net/10453/183677
dc.description.abstract	Zirconium (Zr) is an element commonly used in dental and orthopedic implants either as zirconia (ZrO2) or in metal alloys. It can also be incorporated into calcium silicate-based ceramics. However, the effects of in vitro culture of human osteoblasts (HOBs) with soluble ionic forms of Zr have not been determined. In this study, primary culture of human osteoblasts was conducted in the presence of medium containing either ZrCl4 or Zirconium (IV) oxynitrate (ZrO(NO3)2) at concentrations of 0, 5, 50 and 500 µM, and osteoblast proliferation, differentiation and calcium deposition were assessed. Incubation of human osteoblast cultures with Zr ions increased the proliferation of human osteoblasts and also gene expression of genetic markers of osteoblast differentiation. In 21 and 28 day cultures, Zr ions at concentrations of 50 and 500 µM increased the deposition of calcium phosphate. In addition, the gene expression of BMP2 and BMP receptors was increased in response to culture with Zr ions and this was associated with increased phosphorylation of SMAD1/5. Moreover, Noggin suppressed osteogenic gene expression in HOBs co-treated with Zr ions. In conclusion, Zr ions appear able to induce both the proliferation and the differentiation of primary human osteoblasts. This is associated with up-regulation of BMP2 expression and activation of BMP signaling suggesting this action is, at least in part, mediated by BMP signaling.
dc.format	Electronic-eCollection
dc.language	eng
dc.publisher	PUBLIC LIBRARY SCIENCE
dc.relation.ispartof	PLoS One
dc.relation.isbasedon	10.1371/journal.pone.0113426
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	General Science & Technology
dc.subject.mesh	Bone Morphogenetic Protein 2
dc.subject.mesh	Bone Morphogenetic Protein Receptors
dc.subject.mesh	Bone Morphogenetic Proteins
dc.subject.mesh	Calcification, Physiologic
dc.subject.mesh	Carrier Proteins
dc.subject.mesh	Cell Differentiation
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Chlorides
dc.subject.mesh	Gene Expression Regulation
dc.subject.mesh	Genetic Markers
dc.subject.mesh	Humans
dc.subject.mesh	Osteoblasts
dc.subject.mesh	Osteogenesis
dc.subject.mesh	Signal Transduction
dc.subject.mesh	Smad Proteins
dc.subject.mesh	Zirconium
dc.subject.mesh	Osteoblasts
dc.subject.mesh	Humans
dc.subject.mesh	Chlorides
dc.subject.mesh	Zirconium
dc.subject.mesh	Carrier Proteins
dc.subject.mesh	Bone Morphogenetic Proteins
dc.subject.mesh	Genetic Markers
dc.subject.mesh	Signal Transduction
dc.subject.mesh	Cell Differentiation
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Gene Expression Regulation
dc.subject.mesh	Calcification, Physiologic
dc.subject.mesh	Osteogenesis
dc.subject.mesh	Smad Proteins
dc.subject.mesh	Bone Morphogenetic Protein Receptors
dc.subject.mesh	Bone Morphogenetic Protein 2
dc.subject.mesh	Bone Morphogenetic Protein 2
dc.subject.mesh	Bone Morphogenetic Protein Receptors
dc.subject.mesh	Bone Morphogenetic Proteins
dc.subject.mesh	Calcification, Physiologic
dc.subject.mesh	Carrier Proteins
dc.subject.mesh	Cell Differentiation
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Chlorides
dc.subject.mesh	Gene Expression Regulation
dc.subject.mesh	Genetic Markers
dc.subject.mesh	Humans
dc.subject.mesh	Osteoblasts
dc.subject.mesh	Osteogenesis
dc.subject.mesh	Signal Transduction
dc.subject.mesh	Smad Proteins
dc.subject.mesh	Zirconium
dc.title	Zirconium ions up-regulate the BMP/SMAD signaling pathway and promote the proliferation and differentiation of human osteoblasts.
dc.type	Journal Article
utslib.citation.volume	10
utslib.location.activity	United States
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2025-01-15T20:44:10Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	10
utslib.citation.issue	1

Abstract:

Zirconium (Zr) is an element commonly used in dental and orthopedic implants either as zirconia (ZrO2) or in metal alloys. It can also be incorporated into calcium silicate-based ceramics. However, the effects of in vitro culture of human osteoblasts (HOBs) with soluble ionic forms of Zr have not been determined. In this study, primary culture of human osteoblasts was conducted in the presence of medium containing either ZrCl4 or Zirconium (IV) oxynitrate (ZrO(NO3)2) at concentrations of 0, 5, 50 and 500 µM, and osteoblast proliferation, differentiation and calcium deposition were assessed. Incubation of human osteoblast cultures with Zr ions increased the proliferation of human osteoblasts and also gene expression of genetic markers of osteoblast differentiation. In 21 and 28 day cultures, Zr ions at concentrations of 50 and 500 µM increased the deposition of calcium phosphate. In addition, the gene expression of BMP2 and BMP receptors was increased in response to culture with Zr ions and this was associated with increased phosphorylation of SMAD1/5. Moreover, Noggin suppressed osteogenic gene expression in HOBs co-treated with Zr ions. In conclusion, Zr ions appear able to induce both the proliferation and the differentiation of primary human osteoblasts. This is associated with up-regulation of BMP2 expression and activation of BMP signaling suggesting this action is, at least in part, mediated by BMP signaling.

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http://hdl.handle.net/10453/183677