Faecal metaproteomics analysis reveals a high cardiovascular risk profile across healthy individuals and heart failure patients.

Yang, C; Camargo Tavares, L; Lee, H-C; Steele, JR; Ribeiro, RV; Beale, AL; Yiallourou, S; Carrington, MJ; Kaye, DM; Head, GA; Schittenhelm, RB; Marques, FZ

Faecal metaproteomics analysis reveals a high cardiovascular risk profile across healthy individuals and heart failure patients.

Yang, C Camargo Tavares, L Lee, H-C Steele, JR Ribeiro, RV Beale, AL Yiallourou, S Carrington, MJ Kaye, DM Head, GA Schittenhelm, RB Marques, FZ

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Publisher:: Taylor & Francis
Publication Type:: Journal Article
Citation:: Gut Microbes, 2025, 17, (1), pp. 2441356
Issue Date:: 2025-12

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Field	Value	Language
dc.contributor.author	Yang, C
dc.contributor.author	Camargo Tavares, L
dc.contributor.author	Lee, H-C
dc.contributor.author	Steele, JR
dc.contributor.author	Ribeiro, RV
dc.contributor.author	Beale, AL
dc.contributor.author	Yiallourou, S
dc.contributor.author	Carrington, MJ
dc.contributor.author	Kaye, DM
dc.contributor.author	Head, GA
dc.contributor.author	Schittenhelm, RB
dc.contributor.author	Marques, FZ
dc.date.accessioned	2025-08-06T04:17:30Z
dc.date.available	2025-08-06T04:17:30Z
dc.date.issued	2025-12
dc.identifier.citation	Gut Microbes, 2025, 17, (1), pp. 2441356
dc.identifier.issn	1949-0976
dc.identifier.issn	1949-0984
dc.identifier.uri	http://hdl.handle.net/10453/189209
dc.description.abstract	The gut microbiota is a crucial link between diet and cardiovascular disease (CVD). Using fecal metaproteomics, a method that concurrently captures human gut and microbiome proteins, we determined the crosstalk between gut microbiome, diet, gut health, and CVD. Traditional CVD risk factors (age, BMI, sex, blood pressure) explained < 10% of the proteome variance. However, unsupervised human protein-based clustering analysis revealed two distinct CVD risk clusters (low-risk and high-risk) with different blood pressure (by 9 mmHg) and sex-dependent dietary potassium and fiber intake. In the human proteome, the low-risk group had lower angiotensin-converting enzymes, inflammatory proteins associated with neutrophil extracellular trap formation and auto-immune diseases. In the microbial proteome, the low-risk group had higher expression of phosphate acetyltransferase that produces SCFAs, particularly in fiber-fermenting bacteria. This model identified severity across phenotypes in heart failure patients and long-term risk of cardiovascular events in a large population-based cohort. These findings underscore multifactorial gut-to-host mechanisms that may underlie risk factors for CVD.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Taylor & Francis
dc.relation.ispartof	Gut Microbes
dc.relation.isbasedon	10.1080/19490976.2024.2441356
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0605 Microbiology
dc.subject.classification	3107 Microbiology
dc.subject.mesh	Humans
dc.subject.mesh	Feces
dc.subject.mesh	Gastrointestinal Microbiome
dc.subject.mesh	Male
dc.subject.mesh	Female
dc.subject.mesh	Proteomics
dc.subject.mesh	Middle Aged
dc.subject.mesh	Heart Failure
dc.subject.mesh	Cardiovascular Diseases
dc.subject.mesh	Aged
dc.subject.mesh	Adult
dc.subject.mesh	Proteome
dc.subject.mesh	Bacteria
dc.subject.mesh	Risk Factors
dc.subject.mesh	Diet
dc.subject.mesh	Feces
dc.subject.mesh	Humans
dc.subject.mesh	Bacteria
dc.subject.mesh	Cardiovascular Diseases
dc.subject.mesh	Proteome
dc.subject.mesh	Diet
dc.subject.mesh	Risk Factors
dc.subject.mesh	Proteomics
dc.subject.mesh	Adult
dc.subject.mesh	Aged
dc.subject.mesh	Middle Aged
dc.subject.mesh	Female
dc.subject.mesh	Male
dc.subject.mesh	Heart Failure
dc.subject.mesh	Gastrointestinal Microbiome
dc.subject.mesh	Humans
dc.subject.mesh	Feces
dc.subject.mesh	Gastrointestinal Microbiome
dc.subject.mesh	Male
dc.subject.mesh	Female
dc.subject.mesh	Proteomics
dc.subject.mesh	Middle Aged
dc.subject.mesh	Heart Failure
dc.subject.mesh	Cardiovascular Diseases
dc.subject.mesh	Aged
dc.subject.mesh	Adult
dc.subject.mesh	Proteome
dc.subject.mesh	Bacteria
dc.subject.mesh	Risk Factors
dc.subject.mesh	Diet
dc.title	Faecal metaproteomics analysis reveals a high cardiovascular risk profile across healthy individuals and heart failure patients.
dc.type	Journal Article
utslib.citation.volume	17
utslib.location.activity	United States
utslib.for	0605 Microbiology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Provost
pubs.organisational-group	University of Technology Sydney/Provost/Jumbunna
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2025-08-06T04:17:26Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	17
utslib.citation.issue	1

Abstract:

The gut microbiota is a crucial link between diet and cardiovascular disease (CVD). Using fecal metaproteomics, a method that concurrently captures human gut and microbiome proteins, we determined the crosstalk between gut microbiome, diet, gut health, and CVD. Traditional CVD risk factors (age, BMI, sex, blood pressure) explained < 10% of the proteome variance. However, unsupervised human protein-based clustering analysis revealed two distinct CVD risk clusters (low-risk and high-risk) with different blood pressure (by 9 mmHg) and sex-dependent dietary potassium and fiber intake. In the human proteome, the low-risk group had lower angiotensin-converting enzymes, inflammatory proteins associated with neutrophil extracellular trap formation and auto-immune diseases. In the microbial proteome, the low-risk group had higher expression of phosphate acetyltransferase that produces SCFAs, particularly in fiber-fermenting bacteria. This model identified severity across phenotypes in heart failure patients and long-term risk of cardiovascular events in a large population-based cohort. These findings underscore multifactorial gut-to-host mechanisms that may underlie risk factors for CVD.

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http://hdl.handle.net/10453/189209