Selective separation of microalgae cells using inertial microfluidics

Syed, MS; Rafeie, M; Vandamme, D; Asadnia, M; Henderson, R; Taylor, RA; Warkiani, ME

Selective separation of microalgae cells using inertial microfluidics

Syed, MS Rafeie, M Vandamme, D Asadnia, M Henderson, R Taylor, RA Warkiani, ME

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Publication Type:: Journal Article
Citation:: Bioresource Technology, 2018, 252 pp. 91 - 99
Issue Date:: 2018-03-01

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Field	Value	Language
dc.contributor.author	Syed, MS	en_US
dc.contributor.author	Rafeie, M	en_US
dc.contributor.author	Vandamme, D	en_US
dc.contributor.author	Asadnia, M	en_US
dc.contributor.author	Henderson, R	en_US
dc.contributor.author	Taylor, RA	en_US
dc.contributor.author	Warkiani, ME https://orcid.org/0000-0002-4184-1944	en_US
dc.date.available	2020-05-25T19:15:54Z
dc.date.issued	2018-03-01	en_US
dc.identifier.citation	Bioresource Technology, 2018, 252 pp. 91 - 99	en_US
dc.identifier.issn	0960-8524	en_US
dc.identifier.uri	http://hdl.handle.net/10453/132647
dc.description.abstract	© 2017 Elsevier Ltd Microalgae represent the most promising new source of biomass for the world's growing demands. However, the biomass productivity and quality is significantly decreased by the presence of bacteria or other invading microalgae species in the cultures. We therefore report a low-cost spiral-microchannel that can effectively separate and purify Tetraselmis suecica (lipid-rich microalgae) cultures from Phaeodactylum tricornutum (invasive diatom). Fluorescent polystyrene-microbeads of 6 μm and 10 μm diameters were first used as surrogate particles to optimize the microchannel design by mimicking the microalgae cell behaviour. Using the optimum flowrate, up to 95% of the P. tricornutum cells were separated from the culture without affecting the cell viability. This study shows, for the first time, the potential of inertial microfluidics to sort microalgae species with minimal size difference. Additionally, this approach can also be applied as a pre-sorting technique for water quality analysis.	en_US
dc.relation	http://purl.org/au-research/grants/arc/DP170101773
dc.relation.ispartof	Bioresource Technology	en_US
dc.relation.isbasedon	10.1016/j.biortech.2017.12.065	en_US
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	Biotechnology	en_US
dc.subject.mesh	Diatoms	en_US
dc.subject.mesh	Microfluidics	en_US
dc.subject.mesh	Biomass	en_US
dc.subject.mesh	Chlorophyta	en_US
dc.subject.mesh	Microalgae	en_US
dc.title	Selective separation of microalgae cells using inertial microfluidics	en_US
dc.type	Journal Article
utslib.citation.volume	252	en_US
utslib.for	0904 Chemical Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access	*
pubs.publication-status	Published	en_US
pubs.volume	252	en_US

Abstract:

© 2017 Elsevier Ltd Microalgae represent the most promising new source of biomass for the world's growing demands. However, the biomass productivity and quality is significantly decreased by the presence of bacteria or other invading microalgae species in the cultures. We therefore report a low-cost spiral-microchannel that can effectively separate and purify Tetraselmis suecica (lipid-rich microalgae) cultures from Phaeodactylum tricornutum (invasive diatom). Fluorescent polystyrene-microbeads of 6 μm and 10 μm diameters were first used as surrogate particles to optimize the microchannel design by mimicking the microalgae cell behaviour. Using the optimum flowrate, up to 95% of the P. tricornutum cells were separated from the culture without affecting the cell viability. This study shows, for the first time, the potential of inertial microfluidics to sort microalgae species with minimal size difference. Additionally, this approach can also be applied as a pre-sorting technique for water quality analysis.

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http://hdl.handle.net/10453/132647